neuroligin 2 CRISPR/Cas9 KO Plasmid (h): sc-402250
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- neuroligin 2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the neuroligin 2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
neuroligin 2 CRISPR/Cas9 KO Plasmid (h) | sc-402250 | 20 µg | $397.00 |
Overview
NLGN2 encodes neuroligin 2, a postsynaptic cell-adhesion molecule enriched at inhibitory synapses where it binds presynaptic neurexins to organize synapse formation and maturation. Neuroligin 2 scaffolds GABAergic postsynaptic specialization by coordinating receptor clustering, synaptic signaling, and cytoskeletal organization, influencing excitation–inhibition balance and network synchrony. Through its role in synaptogenesis and synaptic plasticity, NLGN2 intersects with pathways regulating neuronal connectivity and inhibitory neurotransmission. Altered NLGN2 function or expression has been implicated in neurodevelopmental and neuropsychiatric phenotypes associated with disrupted inhibitory circuit development.
neuroligin 2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NLGN2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NLGN2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NLGN2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish neuroligin 2 protein expression.
This CRISPR knockout system enables efficient generation of NLGN2-deficient cell models for investigation of neuroligin 2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NLGN2 exon(s) critical for neuroligin 2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NLGN2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by neuroligin 2 CRISPR/Cas9 KO Plasmid (h) and neuroligin 2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NLGN2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by neuroligin 2 HDR Plasmid (h) and neuroligin 2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NLGN2 homology arms to support homology-directed repair at defined NLGN2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.