Nek9 Antibody (39-7) recommended for detection of Nek9 of mouse, rat and human origin by WB, IP, IF and ELISA

Nek9 Antibody (39-7): sc-100401

Nek9 Antibody (39-7) is rated 4.0 out of 5 by 3.
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Datasheets

    • Anti-Nek9 Antibody (39-7) is a mouse monoclonal IgG2a (kappa light chain) Nek9 antibody provided at 100 µg/ml
    • raised against recombinant Nek9 of human origin
    • recommended for detection of Nek9 of mouse, rat and human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Nek9 (39-7): sc-100401.

I am using Nek9 (39-7): sc-100401 for immunofluorescence, what dilution of this antibody should I use?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good signal strength in Western blot analysis Good signal strength in Western blot analysis of Nek9 expression in HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-20
Rated 4 out of 5 by from Works well with mouse Nek9 transfected 293T and SK Works well with mouse Nek9 transfected 293T and SK-N-MC whole cell lysates for western blot. -SCBT QC
Date published: 2015-04-05
Rated 4 out of 5 by from Produced good immunofluorescence nuclear Produced good immunofluorescence nuclear and cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-03-19
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