NEDL2 CRISPR/Cas9 KO Plasmid (h): sc-410607
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NEDL2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NEDL2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NEDL2 CRISPR/Cas9 KO Plasmid (h) | sc-410607 | 20 µg | $397.00 |
Overview
HECW2 encodes NEDL2, a HECT-type E3 ubiquitin ligase that regulates protein turnover and signaling through ubiquitin-dependent modification of key cellular substrates. NEDL2 activity contributes to proteostasis and influences pathways linked to neuronal development, synaptic function, and stress-responsive transcriptional programs by shaping the stability and availability of regulatory proteins. Disruption or altered regulation of HECW2 has been associated with neurodevelopmental phenotypes and is studied in the context of aberrant ubiquitin signaling that can impact cell-cycle control, differentiation, and genome integrity. As a ubiquitin pathway component, NEDL2 is relevant for mechanistic studies of substrate recognition, E2/E3 network specificity, and downstream effects on signaling nodes.
NEDL2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HECW2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HECW2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HECW2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NEDL2 protein expression.
This CRISPR knockout system enables efficient generation of HECW2-deficient cell models for investigation of NEDL2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HECW2 exon(s) critical for NEDL2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HECW2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NEDL2 CRISPR/Cas9 KO Plasmid (h) and NEDL2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HECW2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NEDL2 HDR Plasmid (h) and NEDL2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HECW2 homology arms to support homology-directed repair at defined HECW2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.