Ndfip2 Antibody (C-6): sc-515327
- Ndfip2 Antibody (C-6) is a mouse monoclonal IgG1 κ Ndfip2 antibody provided at 200 µg/ml
- raised against amino acids 1-150 mapping within an N-terminal cytoplasmic domain of Ndfip2 of mouse origin
- Ndfip2 Antibody (C-6) is recommended for detection of Ndfip2 of mouse origin by WB, IP, IF and ELISA
- Anti-Ndfip2 Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for Ndfip2 Antibody (C-6) for WB applications. This reagent is now offered in a bundle with Ndfip2 Antibody (C-6) (see ordering information below).
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Ndfip2 Antibody (C-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ndfip2 protein of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Ndfip2 antibody (C-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ndfip2, also known as NEDD4 family interacting protein 2 or N4WBP5A, is a 336 amino acid protein that plays a crucial role in cellular processes by localizing to the membranes of the endosome and Golgi apparatus. This localization enables Ndfip2 to participate in endocytosis and regulate key signaling pathways, including NFκB and MAPK, which are vital for cellular responses to stress and inflammation. Ndfip2 interacts with NEDD4 and NEDD4-L, functioning as an adaptor protein that recruits these ubiquitin-protein ligases to the protein trafficking machinery in the Golgi, thereby influencing protein degradation and recycling. Ndfip2 is activated by T cells and can be ubiquitinated by NEDD4 or NEDD4-L, although this modification does not impair function. The gene encoding Ndfip2 is located on human chromosome 13, which contains over 400 genes, including critical tumor suppressor genes such as BRCA2 and RB1, highlighting Ndfip2′s importance in both normal physiology and potential disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ndfip2 Antibody (C-6) References:
- Regulation of the epithelial sodium channel by N4WBP5A, a novel Nedd4/Nedd4-2-interacting protein. | Konstas, AA., et al. 2002. J Biol Chem. 277: 29406-16. PMID: 12050153
- Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways. | Matsuda, A., et al. 2003. Oncogene. 22: 3307-18. PMID: 12761501
- Cloning and characterization of N4WBP5A, an inducible, cyclosporine-sensitive, Nedd4-binding protein in human T lymphocytes. | Cristillo, AD., et al. 2003. J Biol Chem. 278: 34587-97. PMID: 12796489
- Regulation of intestinal phosphate cotransporter NaPi IIb by ubiquitin ligase Nedd4-2 and by serum- and glucocorticoid-dependent kinase 1. | Palmada, M., et al. 2004. Am J Physiol Gastrointest Liver Physiol. 287: G143-50. PMID: 15044175
- N4WBP5A (Ndfip2), a Nedd4-interacting protein, localizes to multivesicular bodies and the Golgi, and has a potential role in protein trafficking. | Shearwin-Whyatt, LM., et al. 2004. J Cell Sci. 117: 3679-89. PMID: 15252135
- Regulation of the divalent metal ion transporter DMT1 and iron homeostasis by a ubiquitin-dependent mechanism involving Ndfips and WWP2. | Foot, NJ., et al. 2008. Blood. 112: 4268-75. PMID: 18776082
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ndfip2 Antibody (C-6) | sc-515327 | 200 µg/ml | $322.00 | |||
Ndfip2 Antibody (C-6): m-IgG Fc BP-HRP Bundle | sc-526431 | 200 µg Ab; 10 µg BP | $361.00 | |||
Ndfip2 Antibody (C-6) AC | sc-515327 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Ndfip2 Antibody (C-6) HRP | sc-515327 HRP | 200 µg/ml | $322.00 | |||
Ndfip2 Antibody (C-6) FITC | sc-515327 FITC | 200 µg/ml | $336.00 | |||
Ndfip2 Antibody (C-6) PE | sc-515327 PE | 200 µg/ml | $349.00 | |||
Ndfip2 Antibody (C-6) Alexa Fluor® 488 | sc-515327 AF488 | 200 µg/ml | $364.00 | |||
Ndfip2 Antibody (C-6) Alexa Fluor® 546 | sc-515327 AF546 | 200 µg/ml | $364.00 | |||
Ndfip2 Antibody (C-6) Alexa Fluor® 594 | sc-515327 AF594 | 200 µg/ml | $364.00 | |||
Ndfip2 Antibody (C-6) Alexa Fluor® 647 | sc-515327 AF647 | 200 µg/ml | $364.00 | |||
Ndfip2 Antibody (C-6) Alexa Fluor® 680 | sc-515327 AF680 | 200 µg/ml | $364.00 | |||
Ndfip2 Antibody (C-6) Alexa Fluor® 790 | sc-515327 AF790 | 200 µg/ml | $364.00 |