NAIP CRISPR/Cas9 KO Plasmid (h): sc-418189
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NAIP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NAIP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NAIP CRISPR/Cas9 KO Plasmid (h) | sc-418189 | 20 µg | $397.00 |
Overview
NAIP (NLR family apoptosis inhibitory protein) is a human cytosolic NOD-like receptor that contributes to innate immune sensing and cell survival regulation. It participates in inflammasome-related processes by recognizing specific bacterial ligands and supporting activation of inflammatory caspase pathways, influencing IL-1 family cytokine maturation and pyroptotic responses. Through these roles, NAIP intersects with NF-κB signaling, host–pathogen defense mechanisms, and cellular stress responses that shape inflammatory homeostasis. Dysregulated NAIP expression or function has been associated with altered inflammatory signaling in infection and immune-mediated pathology, making it a relevant target for mechanistic studies of inflammation and cell death.
NAIP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NAIP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NAIP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NAIP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NAIP protein expression.
This CRISPR knockout system enables efficient generation of NAIP-deficient cell models for investigation of NAIP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NAIP exon(s) critical for NAIP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NAIP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NAIP CRISPR/Cas9 KO Plasmid (h) and NAIP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NAIP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NAIP HDR Plasmid (h) and NAIP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NAIP homology arms to support homology-directed repair at defined NAIP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.