Anti-N-Myc Antibody (3C165) recommended for detection of N-Myc p67 of mouse, rat and human origin by WB, IP and IF

Anti-N-Myc Antibody (3C165): sc-71633

Anti-N-Myc Antibody (3C165) is rated 4.0 out of 5 by 1.
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Datasheets

    • N-Myc Antibody (3C165) is a mouse monoclonal IgG2a, cited in 1 publications, provided at 200 µg/ml
    • epitope mapping at residues 327-339 of N-Myc of human origin
    • recommended for detection of N-Myc p67 of mouse, rat and human origin by WB, IP and IF
    • See N-Myc (B8.4.B): sc-53993 for N-Myc antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for N-Myc Antibody (3C165). This work is in progress.
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How much sample do you recommend loading on the gel for Western blot detection using N-Myc (3C165): sc-71633?

Asked by: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from WB: Good results with human medulloblastoma cells WB: Good results with human medulloblastoma cells. -SCBT Publication Review
Date published: 2010-03-01
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