Nε [γ-glutamyl]-Lysine Antibody (81D1C2): sc-57601

Nε [γ-glutamyl]-Lysine Antibody (81D1C2) is a mouse monoclonal IgG1 antibody that detects Nε [γ-glutamyl]-Lysine in non-species reactive samples through western blotting (WB) and immunofluorescence (IF) applications. Anti-Nε [γ-glutamyl]-Lysine antibody (81D1C2) is available as a non-conjugated antibody, specifically targeting the unique isopeptide bond formed by transglutaminase activity. This bond plays a significant role in various cellular processes, including protein cross-linking and stabilization, which are essential for maintaining structural integrity of proteins. Formation of the Nε [γ-glutamyl]-Lysine bond begins with ubiquitin activation, leading to ubiquitin-adenylate adduct creation and subsequent thiol ester formation, processes vital for cellular signaling and protein regulation. Isopeptide linkages, such as those between N-α-monomethylalanine and lysine, have been observed in ribosomal protein S11 from Escherichia coli, highlighting the importance of these bonds in both prokaryotic and eukaryotic systems. Nε [γ-glutamyl]-Lysine (81D1C2) monoclonal antibody′s ability to detect this specific modification makes this reagent invaluable for studying protein interactions and modifications in various biological contexts.

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Nε [γ-glutamyl]-Lysine Antibody (81D1C2) References:

1. Selective isopeptide bond formation: coupling ubiquitin(67-76) with histone 2A(114-128) by use of the transfer active ester condensation technique.  |  Wang, P., et al. 1999. J Pept Res. 53: 673-7. PMID: 10408342
2. A novel 'clip-and-link' activity of repeat in toxin (RTX) proteins from gram-negative pathogens. Covalent protein cross-linking by an Asp-Lys isopeptide bond upon calcium-dependent processing at an Asp-Pro bond.  |  Osicka, R., et al. 2004. J Biol Chem. 279: 24944-56. PMID: 15044436
3. Synthesis and functional studies of tuftsin analogs containing isopeptide bond.  |  Mezö, G., et al. 1990. Peptides. 11: 405-15. PMID: 2381868
4. Isopeptide linkage between nonhistone and histone 2A polypeptides of chromosomal conjugate-protein A24.  |  Goldknopf, IL. and Busch, H. 1977. Proc Natl Acad Sci U S A. 74: 864-8. PMID: 265581
5. Modification of synthetic peptides related to lactate dehydrogenase (231-242) by protein carboxyl methyltransferase and tyrosine protein kinase: effects of introducing an isopeptide bond between aspartic acid-235 and serine-236.  |  Aswad, DW., et al. 1987. Biochemistry. 26: 675-81. PMID: 3105574
6. Isopeptide linkage between N-alpha-monomethylalanine and lysine in ribosomal protein S11 from Escherichia coli.  |  Chen, R. and Chen-Schmeisser, U. 1977. Proc Natl Acad Sci U S A. 74: 4905-8. PMID: 337304
7. Isopeptide bond formation in epidermis.  |  Kubilus, J. and Baden, HP. 1984. Mol Cell Biochem. 58: 129-37. PMID: 6143250
8. The pancornulins: a group of small proline rich-related cornified envelope precursors with bifunctional capabilities in isopeptide bond formation.  |  Greco, MA., et al. 1995. J Invest Dermatol. 104: 204-10. PMID: 7829876
9. An isopeptide bond splitting enzyme from Hirudo medicinalis similar to gamma-glutamyl transpeptidase.  |  Friedrich, T., et al. 1998. Eur J Biochem. 256: 297-302. PMID: 9760167

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nε [γ-glutamyl]-Lysine Antibody (81D1C2) has 0 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nε [γ-glutamyl]-Lysine Antibody (81D1C2) Specifications

Alternate Product Name: Nε [γ-glutamyl]-Lysine

Product Clone Name: 81D1C2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: Nε

Epitope Species:

Product Reactivity/Detection: Nε [γ-glutamyl]-Lysine bond formed by transglutaminase activity

Species Reactivity/Detection: NSR

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF

Vialing Information: Each vial contains 50 µg IgG₁ in 500 µl of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Nε [γ-glutamyl]-Lysine Antibody (81D1C2): N/A