Mystique Antibody (B-8): sc-515630
- Mystique Antibody (B-8) is a mouse monoclonal IgG1 Mystique antibody provided at 200 µg/ml
- raised against amino acids 14-84 mapping near the N-terminus of Mystique of human origin
- Mystique Antibody (B-8) is recommended for detection of Mystique of human origin by WB, IP, IF and ELISA
- Anti-Mystique Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Mystique Antibody (B-8). This work is in progress.
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Mystique Antibody (B-8) is a mouse monoclonal IgG1 antibody that detects Mystique protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Mystique (B-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Mystique protein, also known as PDLIM2 (PDZ and LIM domain protein 2), PP6345, or SLIM, is a 352 amino acid protein characterized by one LIM zinc-binding domain and one PDZ domain, and exists in four isoforms that localize to either the cytoplasm/cytoskeleton or nucleus. Mystique protein plays a crucial role as an adapter protein that facilitates cell attachment at the actin cytoskeleton, which is vital for maintaining tissue architecture and cellular communication. Mystique protein interacts with specific proteins such as α-actinin-1 and α-actinin-4, contributing to cell-cell interactions and proper migration of epithelial cells. Notably, overexpression of Mystique protein has been linked to suppression of anchorage-independent growth and increased cell adhesion to extracellular matrix components like fibronectin and collagen, which may enhance migratory capabilities of certain tumors. Given Mystique protein′s involvement in promoting tumor migration, research suggests a significant role in progression of breast cancer and other malignant carcinomas, making Mystique protein an important target for research in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Mystique Antibody (B-8) References:
- Global profiling of the cell surface proteome of cancer cells uncovers an abundance of proteins with chaperone function. | Shin, BK., et al. 2003. J Biol Chem. 278: 7607-16. PMID: 12493773
- Large-scale cDNA transfection screening for genes related to cancer development and progression. | Wan, D., et al. 2004. Proc Natl Acad Sci U S A. 101: 15724-9. PMID: 15498874
- Pdlim2, a novel PDZ-LIM domain protein, interacts with alpha-actinins and filamin A. | Torrado, M., et al. 2004. Invest Ophthalmol Vis Sci. 45: 3955-63. PMID: 15505042
- Mystique is a new insulin-like growth factor-I-regulated PDZ-LIM domain protein that promotes cell attachment and migration and suppresses Anchorage-independent growth. | Loughran, G., et al. 2005. Mol Biol Cell. 16: 1811-22. PMID: 15659642
- SLIM is a nuclear ubiquitin E3 ligase that negatively regulates STAT signaling. | Tanaka, T., et al. 2005. Immunity. 22: 729-36. PMID: 15963787
- PDLIM2-mediated termination of transcription factor NF-kappaB activation by intranuclear sequestration and degradation of the p65 subunit. | Tanaka, T., et al. 2007. Nat Immunol. 8: 584-91. PMID: 17468759
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mystique Antibody (B-8) | sc-515630 | 200 µg/ml | $316.00 | |||
Mystique Antibody (B-8) AC | sc-515630 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Mystique Antibody (B-8) HRP | sc-515630 HRP | 200 µg/ml | $316.00 | |||
Mystique Antibody (B-8) FITC | sc-515630 FITC | 200 µg/ml | $330.00 | |||
Mystique Antibody (B-8) PE | sc-515630 PE | 200 µg/ml | $343.00 | |||
Mystique Antibody (B-8) Alexa Fluor® 488 | sc-515630 AF488 | 200 µg/ml | $357.00 | |||
Mystique Antibody (B-8) Alexa Fluor® 546 | sc-515630 AF546 | 200 µg/ml | $357.00 | |||
Mystique Antibody (B-8) Alexa Fluor® 594 | sc-515630 AF594 | 200 µg/ml | $357.00 | |||
Mystique Antibody (B-8) Alexa Fluor® 647 | sc-515630 AF647 | 200 µg/ml | $357.00 | |||
Mystique Antibody (B-8) Alexa Fluor® 680 | sc-515630 AF680 | 200 µg/ml | $357.00 | |||
Mystique Antibody (B-8) Alexa Fluor® 790 | sc-515630 AF790 | 200 µg/ml | $357.00 |