MYEOV2 CRISPR/Cas9 KO Plasmid (h): sc-409378
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MYEOV2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MYEOV2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MYEOV2 CRISPR/Cas9 KO Plasmid (h) | sc-409378 | 20 µg | $397.00 |
Overview
COPS9 encodes a core component of the COP9 signalosome, a multiprotein complex that regulates cullin-RING ubiquitin ligases by controlling cullin neddylation status. Through this activity, COPS9 influences ubiquitin-dependent proteostasis, cell-cycle progression, DNA damage responses, and signal transduction pathways that govern proliferation and differentiation. Disruption of COP9 signalosome function can alter turnover of key regulatory proteins and reshape stress and developmental programs. Aberrant regulation of these processes has been associated with oncogenic phenotypes and other disorders linked to ubiquitin pathway imbalance.
MYEOV2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the COPS9 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the COPS9 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the COPS9 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MYEOV2 protein expression.
This CRISPR knockout system enables efficient generation of COPS9-deficient cell models for investigation of MYEOV2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting COPS9 exon(s) critical for MYEOV2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple COPS9 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MYEOV2 CRISPR/Cas9 KO Plasmid (h) and MYEOV2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the COPS9 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MYEOV2 HDR Plasmid (h) and MYEOV2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by COPS9 homology arms to support homology-directed repair at defined COPS9 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.