MPP1 Antibody (H-7): sc-515194

MPP1 Antibody (H-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects MPP1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MPP1 (H-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MPP1, also known as M-phase phosphoprotein 1 or KIF20B, is a critical motor protein that plays a vital role in cellular processes, particularly during cell division. This 1,820 amino acid protein is characterized by its kinesin-motor domain and is expressed in key tissues such as the kidney, brain, testis, and ovary. MPP1 is essential for proper completion of cytokinesis, as MPP1 facilitates transport of cellular components to the division site, ensuring that daughter cells receive necessary organelles and macromolecules. Additionally, MPP1 interacts with peptidyl-prolyl isomerase Pin1, which is important for regulating protein conformation and function during cell cycle progression. MPP1 is subject to post-translational modifications, including phosphorylation, likely mediated by ATM or ATR, which can influence MPP1′s activity and interactions. With diverse applications and critical role in cellular dynamics, anti-MPP1 antibody (H-7) serves as an invaluable tool for researchers studying cell division and intracellular transport mechanisms.

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MPP1 Antibody (H-7) References:

1. Autoantibodies from patients with idiopathic ataxia bind to M-phase phosphoprotein-1 (MPP1).  |  Fritzler, MJ., et al. 2000. J Investig Med. 48: 28-39. PMID: 10695267
2. Identification of a novel kinesin-related protein, KRMP1, as a target for mitotic peptidyl-prolyl isomerase Pin1.  |  Kamimoto, T., et al. 2001. J Biol Chem. 276: 37520-8. PMID: 11470801
3. M phase phosphoprotein 1 is a human plus-end-directed kinesin-related protein required for cytokinesis.  |  Abaza, A., et al. 2003. J Biol Chem. 278: 27844-52. PMID: 12740395
4. Oncogenic role of MPHOSPH1, a cancer-testis antigen specific to human bladder cancer.  |  Kanehira, M., et al. 2007. Cancer Res. 67: 3276-85. PMID: 17409436
5. The role of MPP1/p55 and its palmitoylation in resting state raft organization in HEL cells.  |  Biernatowska, A., et al. 2013. Biochim Biophys Acta. 1833: 1876-84. PMID: 23507198
6. MPP1 directly interacts with flotillins in erythrocyte membrane - Possible mechanism of raft domain formation.  |  Biernatowska, A., et al. 2017. Biochim Biophys Acta Biomembr. 1859: 2203-2212. PMID: 28865798
7. The microdomain-organizing protein MPP1 is required for insulin-stimulated activation of H-Ras.  |  Podkalicka, J., et al. 2018. Oncotarget. 9: 18410-18421. PMID: 29719614
8. Efficacy of Oral Cryotherapy in the Prevention of Oral Mucositis Associated with Cancer Chemotherapy: Systematic Review with Meta-Analysis and Trial Sequential Analysis.  |  Al-Rudayni, AHM., et al. 2021. Curr Oncol. 28: 2852-2867. PMID: 34436016
9. High-Level Expression of Palmitoylated MPP1 Recombinant Protein in Mammalian Cells.  |  Chytła, A., et al. 2021. Membranes (Basel). 11: PMID: 34564532
10. Single-cell transcriptome analysis of regenerating RGCs reveals potent glaucoma neural repair genes.  |  Li, L., et al. 2022. Neuron. 110: 2646-2663.e6. PMID: 35952672
11. Cloning of cDNAs for M-phase phosphoproteins recognized by the MPM2 monoclonal antibody and determination of the phosphorylated epitope.  |  Westendorf, JM., et al. 1994. Proc Natl Acad Sci U S A. 91: 714-8. PMID: 8290587
12. Identification of novel M phase phosphoproteins by expression cloning.  |  Matsumoto-Taniura, N., et al. 1996. Mol Biol Cell. 7: 1455-69. PMID: 8885239

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MPP1 Antibody (H-7) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-MPP1 Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MPP1 Antibody (H-7) Specifications

Alternate Product Name: MPP1

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1557-1675

Epitope Target: MPP1

Epitope Species: human

Product Reactivity/Detection: MPP1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for MPP1 Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin