MPO/Myeloperoxidase Antibody (266-6K1): sc-52707

MPO Antibody (266-6K1) is a mouse monoclonal IgG1 antibody that detects myeloperoxidase (MPO) of human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-MPO antibody (266-6K1) is available as the non-conjugated format. Myeloperoxidase is a heme-containing enzyme predominantly found in the azurophilic granules of neutrophils and plays a crucial role in the immune response by generating reactive oxygen species, including hypochlorous acid, which are essential for microbial killing. MPO (266-6K1) antibody aids in studying optimal oxygen-dependent microbiocidal activity, as MPO catalyzes the conversion of hydrogen peroxide into potent oxidants, thereby aiding in the destruction of pathogens. The synthesis of MPO begins during the promyelocytic stage of myeloid differentiation, where MPO undergoes cotranslational N-linked glycosylation, resulting in a glycoprotein that is subsequently processed and transported to lysosomes. The final maturation of MPO involves proteolytic cleavage, yielding a functional dimeric enzyme composed of heavy and light chains. Notably, calreticulin, a calcium-binding protein located in the endoplasmic reticulum, specifically interacts with fully glycosylated pro-MPO, highlighting the importance of post-translational modifications in MPO′s maturation and function. MPO is abundantly expressed in circulating neutrophils and monocytes, making MPO a significant marker for inflammatory responses, while MPO remains undetectable in microglia and normal brain tissue, underscoring MPO′s specificity in immune cell populations.

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MPO/Myeloperoxidase Antibody (266-6K1) References:

1. Human hemi-myeloperoxidase. Initial chlorinating activity at neutral pH, compound II and III formation, and stability towards hypochlorous acid and high temperature.  |  Zuurbier, KW., et al. 1992. Eur J Biochem. 205: 737-42. PMID: 1315274
2. The role of myeloperoxidase and myeloperoxidase-antineutrophil cytoplasmic antibodies (MPO-ANCAs) in the pathogenesis of human MPO-ANCA-associated glomerulonephritis.  |  Arimura, Y., et al. 2013. Clin Exp Nephrol. 17: 634-637. PMID: 23504409
3. Preparation and characterization of monoclonal antibodies against human myeloperoxidase.  |  Homma, T., et al. 1989. Arch Biochem Biophys. 273: 189-96. PMID: 2547339
4. Posttranslational processing of a human myeloid lysosomal protein, myeloperoxidase.  |  Nauseef, WM. 1987. Blood. 70: 1143-50. PMID: 2820530
5. Biosynthesis and processing of myeloperoxidase--a marker for myeloid cell differentiation.  |  Nauseef, WM., et al. 1988. Eur J Haematol. 40: 97-110. PMID: 2831080
6. Molecular cloning and characterization of cDNA for human myeloperoxidase.  |  Morishita, K., et al. 1987. J Biol Chem. 262: 3844-51. PMID: 3029127
7. Characterization of cDNA clones for human myeloperoxidase: predicted amino acid sequence and evidence for multiple mRNA species.  |  Johnson, KR., et al. 1987. Nucleic Acids Res. 15: 2013-28. PMID: 3031585
8. The Enzymatic and Non-Enzymatic Function of Myeloperoxidase (MPO) in Inflammatory Communication.  |  Kargapolova, Y., et al. 2021. Antioxidants (Basel). 10: PMID: 33916434
9. MPO-ANCA-positive conversion and microscopic polyangiitis development in idiopathic interstitial pneumonia: a case report.  |  Nakayama, S., et al. 2023. CEN Case Rep. 12: 39-44. PMID: 35749013
10. Therapeutic inhibition of MPO stabilizes pre-existing high risk atherosclerotic plaque.  |  Chen, W., et al. 2022. Redox Biol. 58: 102532. PMID: 36375379
11. Calreticulin functions as a molecular chaperone in the biosynthesis of myeloperoxidase.  |  Nauseef, WM., et al. 1995. J Biol Chem. 270: 4741-7. PMID: 7876246
12. Myeloperoxidase polymorphism is associated with gender specific risk for Alzheimer's disease.  |  Reynolds, WF., et al. 1999. Exp Neurol. 155: 31-41. PMID: 9918702

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MPO/Myeloperoxidase Antibody (266-6K1) has 30 citations in a variety of scientific publications.

Primary antibodies like Anti-MPO/Myeloperoxidase Antibody (266-6K1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MPO/Myeloperoxidase Antibody (266-6K1) Specifications

Alternate Product Name: Myeloperoxidase

Product Clone Name: 266-6K1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: MPO

Epitope Species: human

Product Reactivity/Detection: MPO

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 30 citations in various scientific publications

Available Conjugates/Forms for MPO/Myeloperoxidase Antibody (266-6K1): N/A