MMP-13 Double Nickase Plasmid (m): sc-421670-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MMP-13 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MMP-13 Double Nickase Plasmid (m) and MMP-13 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Mmp13. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MMP-13 Double Nickase Plasmid (m) | sc-421670-NIC | 20 µg | $410.00 | |||
MMP-13 Double Nickase Plasmid (m2) | sc-421670-NIC-2 | 20 µg | $410.00 |
Matrix metalloproteinase-13 (MMP-13), encoded by the mouse Mmp13 gene, is a secreted zinc-dependent endopeptidase that preferentially cleaves fibrillar collagens and other extracellular matrix (ECM) substrates to regulate tissue remodeling. Its expression is induced by inflammatory cytokines and growth factor signaling and interfaces with MAPK/AP-1 and NF-κB–linked transcriptional programs that coordinate ECM turnover and pericellular proteolysis. MMP-13 activity contributes to cartilage and bone matrix dynamics, osteoclast–osteoblast coupling, and wound repair processes through modulation of collagen degradation and downstream matrix-derived signaling cues. Dysregulated Mmp13 is frequently studied in musculoskeletal degeneration, inflammatory tissue remodeling, and tumor–stroma interactions where altered ECM composition influences cell migration and invasion.
MMP-13 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Mmp13 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Mmp13. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Mmp13 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Mmp13-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.