MLTK Antibody (A-2): sc-390924

MLTK Antibody (A-2) is a mouse monoclonal IgM antibody that detects MLTK protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-MLTK antibody (A-2) is available as the non-conjugated form. MLTK, also known as mitogen-activated protein kinase kinase kinase MLT, leucine zipper- and sterile a motif-containing kinase, is an 800 amino acid protein encoded by the human gene ZAK. MLTK functions as a crucial component of cellular signaling pathways, specifically involved in the stress-activated protein kinase cascade, which regulates the c-Jun N-terminal kinase (JNK) and p38 pathways. Such regulation is vital for cellular responses to stress, including apoptosis and cell cycle control. MLTK primarily resides in the cytoplasm as a homodimer but translocates to the nucleus upon ultraviolet B irradiation, highlighting a role in cellular stress responses. MLTK regulates S and G2 cell cycle checkpoints through direct phosphorylation of Chk2, while MLTK isoform 1 associates with cell shrinkage and disruption of actin stress fibers, potentially contributing to neoplastic transformation and cancer development. MLTK isoform 1 phosphorylates Histone H3 at Ser 28, indicating involvement in chromatin remodeling and gene expression regulation.

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MLTK Antibody (A-2) References:

1. Cloning and expression of ZAK, a mixed lineage kinase-like protein containing a leucine-zipper and a sterile-alpha motif.  |  Liu, TC., et al. 2000. Biochem Biophys Res Commun. 274: 811-6. PMID: 10924358
2. Identification and characterization of a novel MAP kinase kinase kinase, MLTK.  |  Gotoh, I., et al. 2001. J Biol Chem. 276: 4276-86. PMID: 11042189
3. Mixed lineage kinase ZAK utilizing MKK7 and not MKK4 to activate the c-Jun N-terminal kinase and playing a role in the cell arrest.  |  Yang, JJ. 2002. Biochem Biophys Res Commun. 297: 105-10. PMID: 12220515
4. A novel zinc finger protein, ZZaPK, interacts with ZAK and stimulates the ZAK-expressing cells re-entering the cell cycle.  |  Yang, JJ. 2003. Biochem Biophys Res Commun. 301: 71-7. PMID: 12535642
5. Regulation of a mitogen-activated protein kinase kinase kinase, MLTK by PKN.  |  Takahashi, M., et al. 2003. J Biochem. 133: 181-7. PMID: 12761180
6. Multiple independent kinase cascades are targeted by hyperosmotic stress but only one activates stress kinase p38.  |  Mao, X., et al. 2004. Exp Cell Res. 292: 304-11. PMID: 14697338
7. A novel role for mixed-lineage kinase-like mitogen-activated protein triple kinase alpha in neoplastic cell transformation and tumor development.  |  Cho, YY., et al. 2004. Cancer Res. 64: 3855-64. PMID: 15172994
8. Phosphorylation of Ser28 in histone H3 mediated by mixed lineage kinase-like mitogen-activated protein triple kinase alpha.  |  Choi, HS., et al. 2005. J Biol Chem. 280: 13545-53. PMID: 15684425
9. A-kinase anchoring protein (AKAP)-Lbc anchors a PKN-based signaling complex involved in α1-adrenergic receptor-induced p38 activation.  |  Cariolato, L., et al. 2011. J Biol Chem. 286: 7925-7937. PMID: 21224381
10. Transient global cerebral ischemia induces up-regulation of MLTKα in hippocampal CA1 neurons.  |  Su, X., et al. 2012. J Mol Histol. 43: 187-93. PMID: 22146853
11. The protein kinase MLTK regulates chondrogenesis by inducing the transcription factor Sox6.  |  Suzuki, T., et al. 2012. Development. 139: 2988-98. PMID: 22764049
12. A-kinase anchoring protein Lbc coordinates a p38 activating signaling complex controlling compensatory cardiac hypertrophy.  |  Pérez López, I., et al. 2013. Mol Cell Biol. 33: 2903-17. PMID: 23716597

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MLTK Antibody (A-2) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-MLTK Antibody (A-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MLTK Antibody (A-2) Specifications

Alternate Product Name: MLTK

Product Clone Name: A-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 211-257

Epitope Target: MLTK

Epitope Species: human

Product Reactivity/Detection: MLTK

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: avian

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for MLTK Antibody (A-2): P: Blocking Peptide