Midline-1 Antibody (2C11): sc-293353

Midline-1 Antibody (2C11) is a mouse monoclonal IgG2b kappa light chain antibody that detects Midline-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Midline-1 antibody (2C11) is available as a non-conjugated antibody. Midline-1 protein, also known as Tripartite motif-containing protein 18, Putative transcription factor XPRF, or RING finger protein 59, is a 667 amino acid protein encoded by the human gene MID1. Midline-1 (2C11) monoclonal antibody recognizes a member of the TRIM/RBCC family featuring a complex structure that includes two B box-type zinc fingers, one B30.2/SPRY domain, one COS domain, one fibronectin type-III domain, and one RING-type zinc finger. The structural composition of Midline-1 is crucial as Midline-1 is believed to possess E3 ubiquitin ligase activity, which plays a significant role in targeting the catalytic subunit of protein phosphatase 2 for degradation, thereby regulating various cellular processes. Midline-1 is primarily located in the cytoplasm and can exist as a homodimer or heterodimer with Midline-2, highlighting Midline-1′s importance in cellular signaling pathways. Midline-1 interacts with IGBP1, a lymphocyte signaling protein, further emphasizing Midline-1′s role in immune response regulation. Defects in the MID1 gene are associated with Opitz syndrome type I, an X-linked recessive disorder characterized by a range of developmental anomalies, including hypertelorism and genital-urinary defects, underscoring the critical functions of Midline-1 in normal development and cellular homeostasis.

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Midline-1 Antibody (2C11) References:

1. The Opitz syndrome gene product, MID1, associates with microtubules.  |  Schweiger, S., et al. 1999. Proc Natl Acad Sci U S A. 96: 2794-9. PMID: 10077590
2. MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development.  |  Buchner, G., et al. 1999. Hum Mol Genet. 8: 1397-407. PMID: 10400986
3. MID1, mutated in Opitz syndrome, encodes an ubiquitin ligase that targets phosphatase 2A for degradation.  |  Trockenbacher, A., et al. 2001. Nat Genet. 29: 287-94. PMID: 11685209
4. MID1 and MID2 homo- and heterodimerise to tether the rapamycin-sensitive PP2A regulatory subunit, alpha 4, to microtubules: implications for the clinical variability of X-linked Opitz GBBB syndrome and other developmental disorders.  |  Short, KM., et al. 2002. BMC Cell Biol. 3: 1. PMID: 11806752
5. Mild phenotypes in a series of patients with Opitz GBBB syndrome with MID1 mutations.  |  So, J., et al. 2005. Am J Med Genet A. 132A: 1-7. PMID: 15558842
6. Solution structure of the RBCC/TRIM B-box1 domain of human MID1: B-box with a RING.  |  Massiah, MA., et al. 2006. J Mol Biol. 358: 532-45. PMID: 16529770
7. Opitz G/BBB syndrome in Xp22: mutations in the MID1 gene cluster in the carboxy-terminal domain.  |  Gaudenz, K., et al. 1998. Am J Hum Genet. 63: 703-10. PMID: 9718340