Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
mGluR-3 Double Nickase Plasmid (h): sc-404012-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- mGluR-3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- mGluR-3 Double Nickase Plasmid (h) and mGluR-3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GRM3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: mGluR-3 Antibody (A-10): sc-271899
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
mGluR-3 Double Nickase Plasmid (h) | sc-404012-NIC | 20 µg | $410.00 | |||
mGluR-3 Double Nickase Plasmid (h2) | sc-404012-NIC-2 | 20 µg | $410.00 |
GRM3 encodes metabotropic glutamate receptor 3 (mGluR-3), a class C GPCR that primarily couples to Gi/o proteins to suppress adenylyl cyclase activity and modulate cAMP-dependent signaling. mGluR-3 regulates synaptic transmission and plasticity by influencing presynaptic neurotransmitter release and shaping postsynaptic excitability, with downstream effects on MAPK/ERK and other second-messenger pathways. In the central nervous system, mGluR-3 is expressed in neuronal and glial populations and contributes to glutamate homeostasis and neuroinflammatory signaling. Genetic and functional studies have linked GRM3-associated signaling to neuropsychiatric and neurodegenerative disease biology, making it a useful target for mechanistic studies of circuit function and cellular stress responses.
mGluR-3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GRM3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GRM3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GRM3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GRM3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.