mGluR-2 Double Nickase Plasmid (h): sc-403054-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- mGluR-2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- mGluR-2 Double Nickase Plasmid (h) and mGluR-2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GRM2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: mGluR-2 Antibody (A-7): sc-271655
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
mGluR-2 Double Nickase Plasmid (h) | sc-403054-NIC | 20 µg | $410.00 | |||
mGluR-2 Double Nickase Plasmid (h2) | sc-403054-NIC-2 | 20 µg | $410.00 |
Human GRM2 encodes the metabotropic glutamate receptor mGluR-2, a class C GPCR that couples primarily to Gi/o proteins to suppress adenylyl cyclase activity and modulate downstream cAMP/PKA signaling. mGluR-2 functions as a key regulator of presynaptic neurotransmitter release and synaptic plasticity, shaping excitatory transmission through modulation of ion channels and neurotransmitter release machinery. Through these mechanisms, GRM2 participates in neuronal network homeostasis and activity-dependent signaling programs relevant to learning and stress responsiveness. Altered GRM2/mGluR-2 signaling has been linked to neuropsychiatric and neurodevelopmental phenotypes, supporting its use in mechanistic studies of glutamatergic circuit dysfunction.
mGluR-2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GRM2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GRM2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GRM2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GRM2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.