Date published: 2026-3-7

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Methyl Blue (CAS 28983-56-4)

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Alternate Names:
Acid Blue 93
Application:
Methyl Blue is a histology stain for collagen in tissue sections
CAS Number:
28983-56-4
Molecular Weight:
799.80
Molecular Formula:
C37H27N3Na2O9S3
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Methyl Blue is a histology dye commonly used to stain collagen blue in tissue sections. The compound has been determined useful as a vital dye for interocular observations. Methyl Blue has been incorporated in Sabouraud agar for the identification of Candida albicans strains. The compound has also been incorporated in other histology staining procedures such as Mallory′s connective tissue stain.


Methyl Blue (CAS 28983-56-4) References

  1. Use of chromogenic tube and methyl blue-sabouraud agar for the identification of Candida albicans strains.  |  Yücesoy, M., et al. 2001. Kobe J Med Sci. 47: 161-7. PMID: 11733655
  2. Analysis and testing of biological stains--the Biological Stain Commission Procedures.  |  Penney, DP., et al. 2002. Biotech Histochem. 77: 237-75. PMID: 12564600
  3. In vivo toxicity testing of methyl blue and aniline blue as vital dyes for intraocular surgery.  |  Thaler, S., et al. 2009. Retina. 29: 1257-65. PMID: 19934820
  4. Methyl blue and aniline blue versus patent blue and trypan blue as vital dyes in cataract surgery: capsule staining properties and cytotoxicity to human cultured corneal endothelial cells.  |  Thaler, S., et al. 2011. J Cataract Refract Surg. 37: 1147-53. PMID: 21596258
  5. Adsorption Performances and Electrochemical Properties of Methyl Blue onto CoFe₂O₄ Nanoparticles.  |  Zhou, XJ., et al. 2021. J Nanosci Nanotechnol. 21: 2203-2211. PMID: 33500035
  6. KIT-6 induced mesostructured TiO2 for photocatalytic degradation of methyl blue.  |  Siraj, Z., et al. 2021. Environ Sci Pollut Res Int. 28: 53340-53352. PMID: 34031833
  7. Efficient adsorption of methyl orange and methyl blue dyes by a novel triptycene-based hyper-crosslinked porous polymer.  |  He, Y., et al. 2022. RSC Adv. 12: 5587-5594. PMID: 35425553
  8. Kinetic adsorption of methyl blue dye from aqueous solution by PVC/PVC-based copolymer containing quaternary amine.  |  Agaguena, A., et al. 2023. Anal Sci. 39: 1371-1383. PMID: 37208560
  9. ZnO/CuSe composite-mediated bandgap modulation for enhanced photocatalytic performance against methyl blue dye.  |  Mubeen, K., et al. 2023. Sci Rep. 13: 19580. PMID: 37949952
  10. Photocatalytic degradation of methyl blue dye with H2O2 sensing.  |  Sharma, P., et al. 2024. RSC Adv. 14: 14606-14615. PMID: 38708118

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Methyl Blue, 50 g

sc-203743
50 g
$83.00

Methyl Blue, 100 g

sc-203743A
100 g
$240.00