Metaxin 3 CRISPR/Cas9 KO Plasmid (h): sc-415611
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Metaxin 3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Metaxin 3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Metaxin 3 CRISPR/Cas9 KO Plasmid (h) | sc-415611 | 20 µg | $397.00 |
Overview
MTX3 encodes metaxin 3, a member of the metaxin family implicated in mitochondrial biology, including the organization of the outer mitochondrial membrane and the biogenesis of mitochondrial protein import complexes. Metaxins are linked to processes such as mitochondrial protein trafficking, maintenance of mitochondrial integrity, and coordination of metabolism with stress responses. Altered mitochondrial homeostasis is a common feature across diverse disease contexts, making MTX3 a useful locus for studying mechanisms that connect mitochondrial dynamics to cell survival and differentiation. Functional interrogation of MTX3 can help define how mitochondrial import pathways influence downstream signaling and energy-dependent cellular programs.
Metaxin 3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MTX3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MTX3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MTX3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Metaxin 3 protein expression.
This CRISPR knockout system enables efficient generation of MTX3-deficient cell models for investigation of Metaxin 3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MTX3 exon(s) critical for Metaxin 3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MTX3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Metaxin 3 CRISPR/Cas9 KO Plasmid (h) and Metaxin 3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MTX3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Metaxin 3 HDR Plasmid (h) and Metaxin 3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MTX3 homology arms to support homology-directed repair at defined MTX3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.