MES hemisodium salt (CAS 117961-21-4)

MES hemisodium salt, with the chemical formula C6H13NO4S·0.5Na, is a derivative of the sulfonic acid buffer MES (2-(N-morpholino)ethanesulfonic acid), widely employed in biochemistry and molecular biology for its buffering capabilities. This buffer is particularly valued for its pH stability in the range of 5.5 to 6.7, making it an optimal choice for maintaining acidic pH conditions in various experimental settings. MES hemisodium salt enhances solubility and ionic strength without significantly altering the pH, which is crucial for maintaining the integrity and activity of biological molecules during experiments. It has been extensively used in research involving enzyme kinetics and other biochemical pathways where precise pH control is necessary to prevent the denaturation of proteins and other macromolecules. Additionally, the buffer is often utilized in the preparation of separation media for electrophoresis and chromatography, providing a stable ionic environment that supports the resolution of biomolecules, such as proteins and nucleic acids. Its role is critical in studies that require a highly controlled environment to prevent interactions that could lead to experimental artifacts. MES hemisodium salt is also used in the cultivation of sensitive cell types in biotechnology, where the gentle buffering capacity helps to maintain the appropriate physiological pH, thereby supporting cellular health and function. This makes it a fundamental component in a variety of research protocols that require a robust yet non-invasive buffering agent.