Mengo 3DPol Antibody (3B7): sc-65633

Mengo 3DPol Antibody (3B7) is a mouse monoclonal IgG1 antibody that detects Mengo 3DPol protein of viral origin by western blotting (WB). Mengo 3DPol (3B7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Mengo 3DPol protein, a crucial component of Mengovirus, plays a significant role in viral replication by synthesizing RNA from the viral genome. This function is vital because RNA polymerase (3Dpol) efficiency directly influences virus replication rate, impacting overall viral load in infected hosts. Understanding Mengo 3DPol activity is essential for studying Mengovirus pathogenesis and developing potential therapeutic strategies, as research can help elucidate how viruses manipulate host cellular machinery to enhance replication. Mengovirus, belonging to the genus Cardiovirus within the Picornaviridae family, is a non-enveloped icosahedral virus with a positive-sense, single-stranded RNA genome divided into three segments, each encoding essential proteins for viral structure and replication. While infections can occur in vertebrates, they typically don′t lead to severe illness, making Mengo 3DPol and host response studies critical for understanding viral behavior.

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Mengo 3DPol Antibody (3B7) References:

1. Quantification of endogenous viral polymerase, 3D(pol), in preparations of Mengo and encephalomyocarditis viruses.  |  Frolov, VG., et al. 1999. Virology. 260: 148-55. PMID: 10405366
2. Mengovirus and encephalomyocarditis virus poly(C) tract lengths can affect virus growth in murine cell culture.  |  Martin, LR., et al. 2000. J Virol. 74: 3074-81. PMID: 10708422
3. Encephalomyocarditis and Mengo viruses productively infect murine T-lymphocyte cell lines but not fresh ex vivo derived T lymphocytes.  |  Neal, ZC., et al. 2002. Viral Immunol. 15: 155-63. PMID: 11952137
4. ANXA2 Facilitates Enterovirus 71 Infection by Interacting with 3D Polymerase and PI4KB to Assist the Assembly of Replication Organelles.  |  Zhang, Q., et al. 2021. Virol Sin. 36: 1387-1399. PMID: 34196914
5. Identification of a novel acylthiourea-based potent broad-spectrum inhibitor for enterovirus 3D polymerase in vitro and in vivo.  |  Liu, X., et al. 2023. Antiviral Res. 213: 105583. PMID: 36965527
6. Correction: Sarry et al. Host-Specific Interplay between Foot-and-Mouth Disease Virus 3D Polymerase and the Type-I Interferon Pathway. Viruses 2023, 15, 666.  |  Sarry, M., et al. 2023. Viruses. 15: PMID: 38005956
7. VP1 codon deoptimization and high-fidelity substitutions in 3D polymerase as potential vaccine strategies for eliciting immune responses against enterovirus A71.  |  Hsieh, W-S., et al. 2024. J Virol. 98: e0155823. PMID: 38174926
8. Attenuated Mengo virus as a vector for immunogenic human immunodeficiency virus type 1 glycoprotein 120.  |  Altmeyer, R., et al. 1994. Proc Natl Acad Sci U S A. 91: 9775-9. PMID: 7937890
9. Genetic dissection of interaction between poliovirus 3D polymerase and viral protein 3AB.  |  Hope, DA., et al. 1997. J Virol. 71: 9490-8. PMID: 9371611
10. The isolation of Mengo virus stable non-capsid polypeptides from infected L cells and preliminary characterization of an RNA polymerase activity associated with polypeptide E.  |  Lund, GA. and Scraba, DG. 1979. J Gen Virol. 44: 391-403. PMID: 230290
11. Mengo virus 3C proteinase: recombinant expression, intergenus substrate cleavage and localization in vivo.  |  Hall, DJ. and Palmenberg, AC. 1996. Virus Genes. 13: 99-110. PMID: 8972564
12. Epitope mapping of monoclonal antibodies raised to recombinant Mengo 3D polymerase.  |  Duque, H. and Palmenberg, AC. 1996. Virus Genes. 13: 159-68. PMID: 8972569

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Mengo 3DPol Antibody (3B7) has 4 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Mengo 3DPol Antibody (3B7) Specifications

Alternate Product Name: Mengo 3DPol

Product Clone Name: 3B7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: Mengo 3DPol

Epitope Species: Mengovirus

Product Reactivity/Detection: Mengo 3D Polymerase

Species Reactivity/Detection: viral

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Mengo 3DPol Antibody (3B7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin