Mel-18 Antibody (B-8): sc-390868
- Mel-18 Antibody (B-8) is a mouse monoclonal mouse IgM κ κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 325-344 at the C-terminus of Mel-18 of human origin
- recommended for detection of Mel-18 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- TransCruz reagent for ChIP application (sc-390868 X, 200 µg/0.1 ml)
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Mel-18 Antibody (B-8) for WB applications. This reagent is now offered in a bundle with Mel-18 Antibody (B-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Mel-18 Antibody (B-8) is a mouse monoclonal IgM antibody that detects Mel-18 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Mel-18, a member of the Polycomb group gene family, plays a crucial role in the regulation of gene expression during development, particularly in the repression of homeotic loci, which is essential for proper embryonic development. Mel-18 (B-8) antibody targets a protein primarily located in the nucleus, where Mel-18 interacts with chromatin to modulate transcriptional activity. Nuclear localization of Mel-18 allows for direct engagement with DNA and associated regulatory elements, thereby influencing the expression of key genes involved in cell proliferation and survival, such as Bcl-2 and c-Myc. Furthermore, Mel-18′s ability to bind DNA through its RING-finger motif facilitates its role as a tumor suppressor, as Mel-18 negatively regulates cell cycle progression and prevents inappropriate entry into the S phase. In contrast, the oncogenic potential of its homolog Bmi-1 highlights the delicate balance of these proteins in cellular processes, making anti-Mel-18 antibody (B-8) a vital tool in understanding both normal development and cancer biology.
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Mel-18 Antibody (B-8) References:
- Contribution of polycomb homologues Bmi-1 and Mel-18 to medulloblastoma pathogenesis. | Wiederschain, D., et al. 2007. Mol Cell Biol. 27: 4968-79. PMID: 17452456
- Mel-18 negatively regulates INK4a/ARF-independent cell cycle progression via Akt inactivation in breast cancer. | Lee, JY., et al. 2008. Cancer Res. 68: 4201-9. PMID: 18519679
- Analysis of Mel-18 expression in prostate cancer tissues and correlation with clinicopathologic features. | Wang, W., et al. 2011. Urol Oncol. 29: 244-51. PMID: 19395284
- The novel tumor-suppressor Mel-18 in prostate cancer: its functional polymorphism, expression and clinical significance. | Wang, W., et al. 2009. Int J Cancer. 125: 2836-43. PMID: 19585577
- BMI1 and Mel-18 oppositely regulate carcinogenesis and progression of gastric cancer. | Zhang, XW., et al. 2010. Mol Cancer. 9: 40. PMID: 20170541
- The binding activity of Mel-18 at the Il17a promoter is regulated by the integrated signals of the TCR and polarizing cytokines. | Hod-Dvorai, R., et al. 2011. Eur J Immunol. 41: 2424-35. PMID: 21674483
- Expression and clinicopathological significance of Mel-18 mRNA in colorectal cancer. | Tao, J., et al. 2014. Tumour Biol. 35: 9619-25. PMID: 24964959
- Polycomb group protein Mel18 inhibits hematopoietic stem cell self-renewal through repressing the transcription of self-renewal and proliferation genes. | Cai, W., et al. 2025. Leukemia. 39: 296-307. PMID: 39562720
- mel-18, a Polycomb group-related mammalian gene, encodes a transcriptional negative regulator with tumor suppressive activity. | Kanno, M., et al. 1995. EMBO J. 14: 5672-8. PMID: 8521824
- A role for mel-18, a Polycomb group-related vertebrate gene, during theanteroposterior specification of the axial skeleton. | Akasaka, T., et al. 1996. Development. 122: 1513-22. PMID: 8625838
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mel-18 Antibody (B-8) | sc-390868 | 200 µg/ml | $322.00 | |||
Mel-18 Antibody (B-8): m-IgGκ BP-HRP Bundle | sc-552544 | 200 µg Ab; 40 µg BP | $361.00 | |||
Mel-18 (B-8) Neutralizing Peptide | sc-390868 P | 100 µg/0.5 ml | $69.00 | |||
Mel-18 Antibody (B-8) X | sc-390868 X | 200 µg/0.1 ml | $322.00 |