MCART1 CRISPR/Cas9 KO Plasmid (h): sc-417948
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MCART1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MCART1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MCART1 CRISPR/Cas9 KO Plasmid (h) | sc-417948 | 20 µg | $397.00 |
Overview
SLC25A51 encodes MCART1, an inner mitochondrial membrane carrier implicated in maintaining mitochondrial redox metabolism by facilitating NAD transport into the matrix. By influencing availability of NAD for oxidative phosphorylation, TCA-cycle-linked dehydrogenase reactions, and mitochondrial sirtuin-dependent protein deacylation, MCART1 contributes to bioenergetic homeostasis and stress responses. Disruption of mitochondrial NAD handling can remodel metabolic signaling and reactive oxygen species balance, processes frequently perturbed in proliferative and degenerative disease contexts. Accordingly, SLC25A51/MCART1 is of interest for studies of mitochondrial function, metabolic rewiring, and genotype-to-phenotype relationships in human cell models.
MCART1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC25A51 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC25A51 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC25A51 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MCART1 protein expression.
This CRISPR knockout system enables efficient generation of SLC25A51-deficient cell models for investigation of MCART1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC25A51 exon(s) critical for MCART1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC25A51 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MCART1 CRISPR/Cas9 KO Plasmid (h) and MCART1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC25A51 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MCART1 HDR Plasmid (h) and MCART1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC25A51 homology arms to support homology-directed repair at defined SLC25A51 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.