Molecular structure of Malantide, Protein Kinase Substrate, CAS Number: 86555-35-3
Malantide, Protein Kinase Substrate (CAS 86555-35-3)
Application:
Malantide, Protein Kinase Substrate is a highly specific PKA substrate
CAS Number:
86555-35-3
Molecular Weight:
1633.8
Molecular Formula:
C72H124N22O21
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
Malantide, Protein Kinase Substrate is a synthetic tetradecapeptide derived from the phosphorylation site of the β-subunit of phosphorylase kinase. the compound is a highly specific substrate for PKA and has displayed PKI inhibition >90% substrate phosphorylation in various tissue extracts. Km=15 μM for PKA and 223 μM for PKG. Malantide is also a PKC substrate and catalyzes the phosphorylation of serine in certain substrates, including troponin I. More recently the compound has been used in studies to directly detect the phosphorylated peptides by electrospray ionization mass spectrometry; shown as a realistic alternative to radioactivity and fluorescence detection in determining enzymatic activity.
Malantide, Protein Kinase Substrate (CAS 86555-35-3) References
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- Selective enrichment of low-abundance peptides in complex mixtures by elution-modified displacement chromatography and their identification by electrospray ionization mass spectrometry. | Wilkins, JA., et al. 2002. Anal Chem. 74: 3933-41. PMID: 12199558
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- Regulation of phosphorylation of tau by protein kinases in rat brain. | Sengupta, A., et al. 2006. Neurochem Res. 31: 1473-80. PMID: 17120162
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- Characterization of a new substrate for protein kinase C: assay by continuous fluorometric monitoring and high performance liquid chromatography. | Zhao, ZH., et al. 1991. Biochem Biophys Res Commun. 176: 1454-61. PMID: 2039524
- Use of a synthetic dodecapeptide (malantide) to measure the cyclic AMP-dependent protein kinase activity ratio in a variety of tissues. | Murray, KJ., et al. 1990. Biochem J. 267: 703-8. PMID: 2160235
- Electrostatic control of peptide side-chain reactivity using amphiphilic homopolymer-based supramolecular assemblies. | Wang, F., et al. 2013. J Am Chem Soc. 135: 14179-88. PMID: 23971726
- Mass spectrometric real-time monitoring of an enzymatic phosphorylation assay using internal standards and data-handling freeware. | Krappmann, M., et al. 2016. Rapid Commun Mass Spectrom. 30: 1019-30. PMID: 27003039
- Peptides derived from MARCKS block coagulation complex assembly on phosphatidylserine. | Kastelowitz, N., et al. 2017. Sci Rep. 7: 4275. PMID: 28655899
- Characterization of a fluorescent substrate for the adenosine 3',5'-cyclic monophosphate-dependent protein kinase. | Malencik, DA. and Anderson, SR. 1983. Anal Biochem. 132: 34-40. PMID: 6312837
- Cyclic AMP-dependent protein kinase activity ratio assay. | Murray, KJ. 1995. Methods Mol Biol. 41: 113-22. PMID: 7655549
- Calcium-Dependent Protein Phosphorylation May Mediate the Gibberellic Acid Response in Barley Aleurone. | Ritchie, S. and Gilroy, S. 1998. Plant Physiol. 116: 765-76. PMID: 9490770
Substrate of:
Enzyme, PKA, and PKC.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Malantide, Protein Kinase Substrate, 1 mg | sc-201154 | 1 mg | $106.00 |