Ly-6K Double Nickase Plasmid (h): sc-404264-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ly-6K Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ly-6K Double Nickase Plasmid (h) and Ly-6K Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LY6K. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ly-6K Antibody (G-11): sc-393560
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ly-6K Double Nickase Plasmid (h) | sc-404264-NIC | 20 µg | $410.00 | |||
Ly-6K Double Nickase Plasmid (h2) | sc-404264-NIC-2 | 20 µg | $410.00 |
LY6K encodes the human Ly-6K glycosylphosphatidylinositol (GPI)-anchored surface protein, a member of the Ly6/uPAR superfamily implicated in cell–cell communication and modulation of membrane-proximal signaling. Ly-6K expression has been associated with changes in epithelial cell behavior, including proliferation, migration, and invasion, consistent with roles in pathways that integrate adhesion dynamics and growth factor signaling at the plasma membrane. Dysregulated LY6K expression is reported across multiple tumor types and is frequently explored as a marker of oncogenic programs and aggressive cellular phenotypes. These properties make LY6K a relevant target for mechanistic studies of tumor biology, membrane signaling regulation, and cancer-associated transcriptional states.
Ly-6K Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LY6K locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LY6K. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LY6K function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LY6K-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.