Lubricin Antibody (1D5): sc-293466

Lubricin Antibody (1D5) is a mouse monoclonal IgG2a kappa light chain antibody that detects Lubricin protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Lubricin antibody (1D5) is available as the non-conjugated isotype antibody. Lubricin, also known as proteoglycan-4 or megakaryocyte stimulating factor, is crucial for maintaining boundary lubrication in articulating joints, which is essential for reducing friction and wear during movement. Lubricin is primarily located in cartilage, liver, and synovial tissue, where Lubricin plays a protective role by inhibiting synovial cell adhesion to cartilage surfaces and preventing protein deposition from synovial fluid into the cartilage matrix. The disulfide-linked dimer structure of Lubricin, formed between cysteine residues 1146 and 1403, is vital for functional integrity and protein cleavage. Notably, defects in the gene encoding Lubricin can lead to Jakobs syndrome, also referred to as camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), an autosomal recessive disorder characterized by joint failure and noninflammatory synoviocyte hyperplasia. Additionally, Lubricin undergoes various levels of glycosylation, which can result in detection at different molecular weights, further emphasizing Lubricin′s complexity and importance in joint health.

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Lubricin Antibody (1D5) References:

1. Lubricin reduces cartilage--cartilage integration.  |  Schaefer, DB., et al. 2004. Biorheology. 41: 503-8. PMID: 15299281
2. The microenvironment around total hip replacement prostheses.  |  Konttinen, YT., et al. 2005. Clin Orthop Relat Res. 28-38. PMID: 15662301
3. The secreted glycoprotein lubricin protects cartilage surfaces and inhibits synovial cell overgrowth.  |  Rhee, DK., et al. 2005. J Clin Invest. 115: 622-31. PMID: 15719068
4. Association of articular cartilage degradation and loss of boundary-lubricating ability of synovial fluid following injury and inflammatory arthritis.  |  Elsaid, KA., et al. 2005. Arthritis Rheum. 52: 1746-55. PMID: 15934070
5. Consequences of disease-causing mutations on lubricin protein synthesis, secretion, and post-translational processing.  |  Rhee, DK., et al. 2005. J Biol Chem. 280: 31325-32. PMID: 16000300
6. Immunolocalization of lubricin in the rat periodontal ligament during experimental tooth movement.  |  Leonardi, R., et al. 2012. Acta Histochem. 114: 700-4. PMID: 22209395
7. Lubricin in human temporomandibular joint disc: an immunohistochemical study.  |  Leonardi, R., et al. 2012. Arch Oral Biol. 57: 614-9. PMID: 22244189
8. Anti-lubricin monoclonal antibodies created using lubricin-knockout mice immunodetect lubricin in several species and in patients with healthy and diseased joints.  |  Ai, M., et al. 2015. PLoS One. 10: e0116237. PMID: 25642942
9. Lubricin/Proteoglycan 4 Binding to CD44 Receptor: A Mechanism of the Suppression of Proinflammatory Cytokine-Induced Synoviocyte Proliferation by Lubricin.  |  Al-Sharif, A., et al. 2015. Arthritis Rheumatol. 67: 1503-13. PMID: 25708025
10. Lubricin Distribution in the Menisci and Labra of Human Osteoarthritic Joints.  |  Zhang, D., et al. 2012. Cartilage. 3: 165-72. PMID: 26069629
11. Abundant lubricin expression suggests a link between synoviocytes, synovial tumors, and myxomas.  |  Solka, KA., et al. 2016. Histol Histopathol. 31: 1131-41. PMID: 26924731
12. Sialidase Unmasks Mucin Domain Epitopes of Lubricin.  |  Solka, KA., et al. 2016. J Histochem Cytochem. 64: 647-668. PMID: 27680668