LST1 CRISPR/Cas9 KO Plasmid (m): sc-421476
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LST1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LST1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LST1 CRISPR/Cas9 KO Plasmid (m) | sc-421476 | 20 µg | $397.00 |
Overview
Lst1 encodes leukocyte specific transcript 1 (LST1), a small immunoregulatory membrane-associated protein enriched in myeloid lineages such as monocytes, macrophages, and microglia. LST1 participates in shaping innate immune cell activation, migration, and cell–cell communication, with reported links to ITAM-associated signaling networks, cytoskeletal remodeling, and inflammatory transcriptional programs. In mouse tissues, Lst1 expression tracks with macrophage and microglial states and is commonly used as a marker of immune infiltration and myeloid activation. Altered LST1-associated myeloid signatures have been observed across inflammatory and neuroinflammatory contexts and in tumor immune microenvironments, supporting mechanistic studies of immune regulation and disease-relevant myeloid phenotypes.
LST1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Lst1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Lst1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Lst1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LST1 protein expression.
This CRISPR knockout system enables efficient generation of Lst1-deficient cell models for investigation of LST1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Lst1 exon(s) critical for LST1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Lst1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LST1 CRISPR/Cas9 KO Plasmid (m) and LST1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Lst1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LST1 HDR Plasmid (m) and LST1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Lst1 homology arms to support homology-directed repair at defined Lst1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.