LRP6 Double Nickase Plasmid (h): sc-400844-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LRP6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LRP6 Double Nickase Plasmid (h) and LRP6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LRP6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LRP6 Antibody (C-10): sc-25317
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LRP6 Double Nickase Plasmid (h) | sc-400844-NIC | 20 µg | $410.00 | |||
LRP6 Double Nickase Plasmid (h2) | sc-400844-NIC-2 | 20 µg | $410.00 |
LRP6 (low-density lipoprotein receptor–related protein 6) is a single-pass transmembrane co-receptor that couples WNT ligands and Frizzled receptors to activate canonical WNT/β-catenin signaling. Through ligand-dependent phosphorylation and recruitment of AXIN, LRP6 regulates β-catenin stabilization, transcriptional programs controlling proliferation, differentiation, and tissue homeostasis, and crosstalk with pathways governing cell polarity and metabolism. Dysregulated LRP6 activity or expression has been linked to altered WNT signaling dynamics observed across developmental disorders and multiple disease contexts, including cancer biology and metabolic phenotypes. As a result, LRP6 is widely studied for its roles in receptor complex assembly, endocytosis-associated signaling control, and transcriptional output of β-catenin/TCF target genes.
LRP6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LRP6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LRP6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LRP6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LRP6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.