LAL Antibody (9G7F12): sc-58374

LAL Antibody (9G7F12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Lysosomal Acid Lipase (LAL) protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-LAL antibody (9G7F12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LAL protein, a member of the lipase gene family, plays a vital role in lipid metabolism, specifically in the hydrolysis of triglycerides and cholesteryl esters within lysosomes. LAL enzymatic activity is crucial for maintaining lipid homeostasis, and defects in LAL gene can lead to severe metabolic disorders such as Wolman disease and cholesteryl ester storage disease. LAL has six potential N-glycosylation sites and one potential O-glycosylation site, which are important for stability and function, as these post-translational modifications can influence enzyme activity and substrate recognition. LAL overexpression has also been implicated in the development of atherosclerosis, highlighting significance in both normal physiology and disease states.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

LAL Antibody (9G7F12) References:

1. Lysosomal acid lipase-deficient mice: depletion of white and brown fat, severe hepatosplenomegaly, and shortened life span.  |  Du, H., et al. 2001. J Lipid Res. 42: 489-500. PMID: 11290820
2. Enzyme therapy for lysosomal acid lipase deficiency in the mouse.  |  Du, H., et al. 2001. Hum Mol Genet. 10: 1639-48. PMID: 11487567
3. The alpha/beta hydrolase fold.  |  Ollis, DL., et al. 1992. Protein Eng. 5: 197-211. PMID: 1409539
4. Production of recombinant human lysosomal acid lipase in Schizosaccharomyces pombe: development of a fed-batch fermentation and purification process.  |  Ikeda, S., et al. 2004. J Biosci Bioeng. 98: 366-73. PMID: 16233721
5. Treatment of dyslipidemia with lovastatin and ezetimibe in an adolescent with cholesterol ester storage disease.  |  Tadiboyina, VT., et al. 2005. Lipids Health Dis. 4: 26. PMID: 16255772
6. TRP-ML1 regulates lysosomal pH and acidic lysosomal lipid hydrolytic activity.  |  Soyombo, AA., et al. 2006. J Biol Chem. 281: 7294-301. PMID: 16361256
7. The role of mannosylated enzyme and the mannose receptor in enzyme replacement therapy.  |  Du, H., et al. 2005. Am J Hum Genet. 77: 1061-74. PMID: 16380916
8. Each lipase has a unique sensitivity profile for organophosphorus inhibitors.  |  Quistad, GB., et al. 2006. Toxicol Sci. 91: 166-72. PMID: 16449251
9. Autophagy regulates cholesterol efflux from macrophage foam cells via lysosomal acid lipase.  |  Ouimet, M., et al. 2011. Cell Metab. 13: 655-67. PMID: 21641547
10. Lysosomal Acid Lipase in Lipid Metabolism and Beyond.  |  Li, F. and Zhang, H. 2019. Arterioscler Thromb Vasc Biol. 39: 850-856. PMID: 30866656
11. Recent insights into lysosomal acid lipase deficiency.  |  Korbelius, M., et al. 2023. Trends Mol Med. 29: 425-438. PMID: 37028992
12. Targeted disruption of the mouse lysosomal acid lipase gene: long-term survival with massive cholesteryl ester and triglyceride storage.  |  Du, H., et al. 1998. Hum Mol Genet. 7: 1347-54. PMID: 9700186

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LAL Antibody (9G7F12) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-LAL Antibody (9G7F12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LAL Antibody (9G7F12) Specifications

Alternate Product Name: LAL

Product Clone Name: 9G7F12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: LAL

Epitope Species: human

Product Reactivity/Detection: Lipase A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for LAL Antibody (9G7F12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin