Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
L-type Ca++ CP β2 Double Nickase Plasmid (h): sc-403900-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- L-type Ca++ CP β2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- L-type Ca++ CP β2 Double Nickase Plasmid (h) and L-type Ca++ CP β2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CACNB2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: L-type Ca++ CP β2 Antibody (F-29): sc-81890
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
L-type Ca++ CP β2 Double Nickase Plasmid (h) | sc-403900-NIC | 20 µg | $410.00 | |||
L-type Ca++ CP β2 Double Nickase Plasmid (h2) | sc-403900-NIC-2 | 20 µg | $410.00 |
CACNB2 encodes the β2 auxiliary subunit of L-type voltage-gated calcium channels, regulating channel trafficking, membrane expression, and gating kinetics that shape Ca2+ influx during depolarization. By tuning CaV1 channel activity, CACNB2 influences excitation–contraction coupling, synaptic transmission, and activity-dependent gene regulation through calcium-sensitive pathways such as CaMK and calcineurin/NFAT signaling. Altered CACNB2 function has been associated with electrical and signaling abnormalities in excitable tissues and is frequently studied in the context of arrhythmia susceptibility, neuropsychiatric phenotypes, and other calcium channel–related disorders. As an accessory subunit, β2 also impacts pharmacologic sensitivity and biophysical properties of L-type Ca2+ currents, making it relevant for mechanistic studies of channelopathies.
L-type Ca++ CP β2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CACNB2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CACNB2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CACNB2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CACNB2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.