Ksr-2 Antibody (K75): sc-100421

Ksr-2 Antibody (K75) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ksr-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Ksr-2 antibody (K75) is available as the non-conjugated format. Ksr-2 (K75) antibody recognizes a protein that plays a crucial role in regulating the Ras signaling pathway, essential for various cellular processes, including cell growth, differentiation, and survival. Located primarily in the cytoplasm, Ksr-2 acts as a scaffold that facilitates interaction between different components of the MAP kinase pathway, specifically Raf, MEK, and ERK. This spatial organization ensures efficient transmission of signals from Ras to downstream effectors, influencing cellular responses to growth factors and other stimuli. Ksr-2 is necessary for Ras-mediated signaling during germline meiotic progression and functions redundantly with Ksr-1 in developing critical structures such as the excretory system pathway, hermaphrodite vulva, and male spicules. Ksr-2 (K75) monoclonal antibody targets a protein that serves as a negative regulator of MEKK3-mediated activation of MAP kinase pathways, including ERK and JNK, as well as NFκB pathways, playing a significant role in modulating inflammatory responses by inhibiting MEKK3-mediated interleukin-8 production.

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Ksr-2 Antibody (K75) References:

1. Kinase suppressor of Ras forms a multiprotein signaling complex and modulates MEK localization.  |  Stewart, S., et al. 1999. Mol Cell Biol. 19: 5523-34. PMID: 10409742
2. C. elegans ksr-1 and ksr-2 have both unique and redundant functions and are required for MPK-1 ERK phosphorylation.  |  Ohmachi, M., et al. 2002. Curr Biol. 12: 427-33. PMID: 11882296
3. Identification of a novel human kinase supporter of Ras (hKSR-2) that functions as a negative regulator of Cot (Tpl2) signaling.  |  Channavajhala, PL., et al. 2003. J Biol Chem. 278: 47089-97. PMID: 12975377
4. hKSR-2 inhibits MEKK3-activated MAP kinase and NF-kappaB pathways in inflammation.  |  Channavajhala, PL., et al. 2005. Biochem Biophys Res Commun. 334: 1214-8. PMID: 16039990
5. The KSR2-calcineurin complex regulates STIM1-ORAI1 dynamics and store-operated calcium entry (SOCE).  |  Giurisato, E., et al. 2014. Mol Biol Cell. 25: 1769-81. PMID: 24672054
6. Kinase Suppressor of Ras 2 (KSR2) expression in the brain regulates energy balance and glucose homeostasis.  |  Guo, L., et al. 2017. Mol Metab. 6: 194-205. PMID: 28180061
7. KSR2-14-3-3ζ complex serves as a biomarker and potential therapeutic target in sorafenib-resistant hepatocellular carcinoma.  |  Gao, C., et al. 2022. Biomark Res. 10: 25. PMID: 35468812
8. The C. elegans ksr-1 gene encodes a novel Raf-related kinase involved in Ras-mediated signal transduction.  |  Sundaram, M. and Han, M. 1995. Cell. 83: 889-901. PMID: 8521513
9. Murine Ksr interacts with MEK and inhibits Ras-induced transformation.  |  Denouel-Galy, A., et al. 1998. Curr Biol. 8: 46-55. PMID: 9427625
10. Regulation of the MAP kinase pathway by mammalian Ksr through direct interaction with MEK and ERK.  |  Yu, W., et al. 1998. Curr Biol. 8: 56-64. PMID: 9427629
11. The kinase suppressor of Ras (KSR) modulates growth factor and Ras signaling by uncoupling Elk-1 phosphorylation from MAP kinase activation.  |  Sugimoto, T., et al. 1998. EMBO J. 17: 1717-27. PMID: 9501093
12. Kinase suppressor of Ras inhibits the activation of extracellular ligand-regulated (ERK) mitogen-activated protein (MAP) kinase by growth factors, activated Ras, and Ras effectors.  |  Joneson, T., et al. 1998. J Biol Chem. 273: 7743-8. PMID: 9516483

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ksr-2 Antibody (K75) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Ksr-2 Antibody (K75) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ksr-2 Antibody (K75) Specifications

Alternate Product Name: Ksr-2

Product Clone Name: K75

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 411-500

Epitope Target: Ksr-2

Epitope Species: human

Product Reactivity/Detection: Ksr-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Ksr-2 Antibody (K75): N/A