KIF4A CRISPR/Cas9 KO Plasmid (h): sc-411788
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KIF4A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KIF4A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KIF4A CRISPR/Cas9 KO Plasmid (h) | sc-411788 | 20 µg | $397.00 |
Overview
KIF4A encodes a kinesin family motor protein that associates with microtubules and chromatin to coordinate mitotic spindle organization, chromosome condensation, and anaphase segregation. It functions in key cell-cycle processes including cytokinesis and regulation of spindle midzone dynamics, supporting faithful genome partitioning during proliferative division. KIF4A activity intersects with DNA damage response and chromatin remodeling pathways that influence genome stability and replication stress outcomes. Dysregulated KIF4A expression has been linked to proliferative phenotypes and genomic instability signatures observed across multiple cancer research contexts, making it a relevant target for mechanistic studies of mitosis-associated vulnerability.
KIF4A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KIF4A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KIF4A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KIF4A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KIF4A protein expression.
This CRISPR knockout system enables efficient generation of KIF4A-deficient cell models for investigation of KIF4A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KIF4A exon(s) critical for KIF4A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KIF4A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KIF4A CRISPR/Cas9 KO Plasmid (h) and KIF4A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KIF4A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KIF4A HDR Plasmid (h) and KIF4A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KIF4A homology arms to support homology-directed repair at defined KIF4A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.