KCTD5 CRISPR/Cas9 KO Plasmid (h): sc-409961
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KCTD5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KCTD5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KCTD5 CRISPR/Cas9 KO Plasmid (h) | sc-409961 | 20 µg | $397.00 |
Overview
KCTD5 encodes a potassium channel tetramerization domain–containing protein that functions as an adaptor in ubiquitin-dependent protein homeostasis, commonly acting with CUL3-based E3 ligase complexes to influence substrate ubiquitination and turnover. Through these interactions, KCTD5 can impact signaling network dynamics and cellular proteostasis, including processes linked to neuronal function and stress-responsive pathways. Altered regulation of KCTD family adaptors has been associated with dysregulated protein degradation and perturbed signaling outputs, which are recurrent features in cancer biology and neurobiological disorders. Consequently, KCTD5 is studied as a modulatory node connecting ubiquitination machinery to pathway control and cellular phenotype.
KCTD5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KCTD5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KCTD5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KCTD5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KCTD5 protein expression.
This CRISPR knockout system enables efficient generation of KCTD5-deficient cell models for investigation of KCTD5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KCTD5 exon(s) critical for KCTD5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KCTD5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KCTD5 CRISPR/Cas9 KO Plasmid (h) and KCTD5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KCTD5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KCTD5 HDR Plasmid (h) and KCTD5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KCTD5 homology arms to support homology-directed repair at defined KCTD5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.