Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
KCNQ2 Double Nickase Plasmid (h): sc-402492-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KCNQ2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- KCNQ2 Double Nickase Plasmid (h) and KCNQ2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KCNQ2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: KCNQ2 Antibody (C-4): sc-271852
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KCNQ2 Double Nickase Plasmid (h) | sc-402492-NIC | 20 µg | $410.00 | |||
KCNQ2 Double Nickase Plasmid (h2) | sc-402492-NIC-2 | 20 µg | $410.00 |
KCNQ2 encodes a voltage-gated potassium channel subunit that assembles into neuronal M-type (Kv7) currents, shaping resting membrane potential and regulating action potential firing. By controlling subthreshold excitability and spike-frequency adaptation, KCNQ2 integrates into signaling processes that couple membrane voltage dynamics with neurotransmission and network stability. Altered KCNQ2 function has been associated with neurodevelopmental and epilepsy-related phenotypes, making it a key target for dissecting mechanisms of excitability and synaptic circuit regulation in human cell models. Research applications commonly include studying ion channel biophysics, neuronal differentiation outcomes, and pathway-level changes in activity-dependent gene expression.
KCNQ2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KCNQ2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KCNQ2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KCNQ2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KCNQ2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.