KCC1 CRISPR/Cas9 KO Plasmid (m): sc-422973
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KCC1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KCC1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KCC1 CRISPR/Cas9 KO Plasmid (m) | sc-422973 | 20 µg | $397.00 |
Overview
Slc12a4 encodes the mouse K–Cl cotransporter KCC1 (SLC12A4), an electroneutral transporter that couples K+ and Cl− efflux to regulate intracellular chloride, cell volume, and ionic homeostasis. KCC1 activity contributes to volume-regulatory decrease pathways, osmotic stress responses, and chloride-dependent signaling that can influence membrane potential and cytoskeletal dynamics. In hematopoietic and immune lineages, KCC1 supports ion flux programs linked to erythrocyte hydration and leukocyte activation states, providing mechanistic context for studying transport-driven physiology. Dysregulated K–Cl transport has been associated with altered red blood cell properties and broader inflammatory or metabolic phenotypes, making Slc12a4 a useful node for ion transport and homeostasis research.
KCC1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Slc12a4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Slc12a4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Slc12a4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KCC1 protein expression.
This CRISPR knockout system enables efficient generation of Slc12a4-deficient cell models for investigation of KCC1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Slc12a4 exon(s) critical for KCC1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Slc12a4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KCC1 CRISPR/Cas9 KO Plasmid (m) and KCC1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Slc12a4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KCC1 HDR Plasmid (m) and KCC1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Slc12a4 homology arms to support homology-directed repair at defined Slc12a4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.