Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
karyopherin α3 CRISPR/Cas9 KO Plasmid (h): sc-410855
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- karyopherin α3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the karyopherin α3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: karyopherin α3 Antibody (B-1): sc-514101
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
karyopherin α3 CRISPR/Cas9 KO Plasmid (h) | sc-410855 | 20 µg | $397.00 |
Overview
KPNA3 encodes karyopherin α3, an importin-α family adaptor that recognizes classical nuclear localization signals and assembles with importin β to mediate Ran GTPase–dependent nucleocytoplasmic transport through the nuclear pore complex. By regulating nuclear entry of transcription factors, signaling mediators, and other cargo proteins, KPNA3 influences gene expression programs, cell cycle control, stress responses, and innate immune signaling. Altered nuclear transport dynamics involving importin pathways have been associated with dysregulated proliferation, inflammatory signaling, and host–pathogen interactions in multiple disease contexts. KPNA3 is therefore a useful target for dissecting how selective cargo import shapes cellular phenotypes and signal transduction networks.
karyopherin α3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KPNA3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KPNA3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KPNA3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish karyopherin α3 protein expression.
This CRISPR knockout system enables efficient generation of KPNA3-deficient cell models for investigation of karyopherin α3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KPNA3 exon(s) critical for karyopherin α3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KPNA3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by karyopherin α3 CRISPR/Cas9 KO Plasmid (h) and karyopherin α3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KPNA3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by karyopherin α3 HDR Plasmid (h) and karyopherin α3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KPNA3 homology arms to support homology-directed repair at defined KPNA3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.