
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
karyopherin α3 CRISPR/Cas9 KO Plasmid (h) | sc-410855 | 20 µg | $397.00 |
KPNA3 encodes karyopherin α3, an importin-α family adaptor that recognizes classical nuclear localization signals and assembles with importin β to mediate Ran GTPase–dependent nucleocytoplasmic transport through the nuclear pore complex. By regulating nuclear entry of transcription factors, signaling mediators, and other cargo proteins, KPNA3 influences gene expression programs, cell cycle control, stress responses, and innate immune signaling. Altered nuclear transport dynamics involving importin pathways have been associated with dysregulated proliferation, inflammatory signaling, and host–pathogen interactions in multiple disease contexts. KPNA3 is therefore a useful target for dissecting how selective cargo import shapes cellular phenotypes and signal transduction networks.
karyopherin α3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KPNA3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KPNA3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KPNA3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish karyopherin α3 protein expression.
This CRISPR knockout system enables efficient generation of KPNA3-deficient cell models for investigation of karyopherin α3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
CRISPRs +/- HDRs
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.