Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
karyopherin 13 CRISPR/Cas9 KO Plasmid (h): sc-404551
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- karyopherin 13 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the karyopherin 13 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: karyopherin 13 Antibody (D-3): sc-271218
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
karyopherin 13 CRISPR/Cas9 KO Plasmid (h) | sc-404551 | 20 µg | $397.00 |
Overview
IPO13 encodes karyopherin 13 (importin-13), a bidirectional nuclear transport receptor that mediates RanGTP-dependent shuttling of specific protein cargos across the nuclear pore complex. By controlling nuclear import/export dynamics, IPO13 influences transcriptional programs, cell-cycle progression, and stress-responsive signaling through regulation of cargo localization and availability. Altered nucleocytoplasmic transport is a recurring feature of oncogenic transformation and neurodegenerative phenotypes, making IPO13 a relevant node for studying how transport fidelity shapes genome regulation and proteostasis. IPO13 function is also pertinent to host–pathogen interactions and innate immune signaling where nuclear access of regulatory factors can be rate limiting.
karyopherin 13 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the IPO13 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the IPO13 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the IPO13 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish karyopherin 13 protein expression.
This CRISPR knockout system enables efficient generation of IPO13-deficient cell models for investigation of karyopherin 13 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting IPO13 exon(s) critical for karyopherin 13 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple IPO13 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by karyopherin 13 CRISPR/Cas9 KO Plasmid (h) and karyopherin 13 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the IPO13 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by karyopherin 13 HDR Plasmid (h) and karyopherin 13 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by IPO13 homology arms to support homology-directed repair at defined IPO13 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.