JAB1 Antibody (42) recommended for detection of JAB1 of mouse, rat and human origin by WB, IP and IF

JAB1 Antibody (42): sc-135954

JAB1 Antibody (42) is rated 4.0 out of 5 by 2.
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Datasheets

    • JAB1 Antibody (42) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
    • raised against amino acids 234-334 of JAB1 of human origin
    • recommended for detection of JAB1 of mouse, rat and human origin by WB, IP and IF
    • See JAB1 (B-7): sc-13157 for JAB1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

I am using JAB1 (42): sc-135954 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jenni
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Nice band seen in WB using Jurkat whole cell Nice band seen in WB using Jurkat whole cell lysate. -SCBT QC
Date published: 2015-05-25
Rated 4 out of 5 by from Robust immunofluorescence nuclear and cytoplasmic Robust immunofluorescence nuclear and cytoplasmic staining in A-431 cells. -SCBT QC
Date published: 2015-05-04
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