Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IRX3 CRISPR/Cas9 KO Plasmid (h): sc-404632
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IRX3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IRX3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IRX3 Antibody (G-6): sc-166877
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IRX3 CRISPR/Cas9 KO Plasmid (h) | sc-404632 | 20 µg | $397.00 |
Overview
IRX3 (Iroquois homeobox 3) encodes a homeobox transcription factor that helps establish transcriptional programs during embryonic patterning and organogenesis, with context-dependent roles in cell fate specification and differentiation. In human cells, IRX3 integrates with developmental signaling networks, including pathways linked to Wnt and BMP signaling, to shape lineage decisions and tissue-specific gene expression. Beyond development, altered IRX3 regulation has been associated with metabolic phenotypes and changes in adipocyte biology, and dysregulated IRX3 expression has been reported across multiple cancer contexts. These features make IRX3 a useful node for studying transcriptional control, differentiation state, and pathway-driven gene regulatory circuitry.
IRX3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the IRX3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the IRX3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the IRX3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IRX3 protein expression.
This CRISPR knockout system enables efficient generation of IRX3-deficient cell models for investigation of IRX3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting IRX3 exon(s) critical for IRX3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple IRX3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IRX3 CRISPR/Cas9 KO Plasmid (h) and IRX3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the IRX3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IRX3 HDR Plasmid (h) and IRX3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by IRX3 homology arms to support homology-directed repair at defined IRX3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.