IP3KA Antibody (F-3): sc-271838
- IP3KA Antibody (F-3) is a mouse monoclonal IgG1 κ IP3KA antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-25 at the N-terminus of IP3KA of human origin
- IP3KA Antibody (F-3) is recommended for detection of IP3KA of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-IP3KA Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for IP3KA Antibody (F-3) for WB applications. This reagent is now offered in a bundle with IP3KA Antibody (F-3) (see ordering information below).
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IP3KA Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects IP3KA protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IP3KA (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IP3KA plays a crucial role in cellular signaling by regulating intracellular calcium levels through the hydrolysis of inositol 1,4,5-trisphosphate (Ins(1,4,5)P3), which releases calcium from endoplasmic reticulum stores. This calcium release is vital for numerous physiological processes, including muscle contraction, neurotransmitter release, and cell proliferation. IP3KA is involved in the phosphorylation of Ins(1,4,5)P3 to form inositol 1,3,4,5-tetrakisphosphate (Ins(1,4,5)P4), which modulates calcium influx across the plasma membrane, thereby influencing various signaling pathways. IP3KA exists in three isoforms, with IP3KA being the most extensively studied, particularly in rat brain and testis, while IP3KB is expressed in various tissues, including lung and heart. IP3KA activity is enhanced by calmodulin and regulated through phosphorylation by several kinases, which underscores its importance in the inositol phosphate signaling pathways that govern cellular responses to external stimuli.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
IP3KA Antibody (F-3) References:
- Inositol tetrakisphosphate as a second messenger: confusions, contradictions, and a potential resolution. | Irvine, RF. 1991. Bioessays. 13: 419-27. PMID: 1659392
- Regulation of D-myo-inositol 1,4,5-trisphosphate 3-kinase by cAMP-dependent protein kinase and protein kinase C. | Sim, SS., et al. 1990. J Biol Chem. 265: 10367-72. PMID: 2162347
- Cloning and expression in Escherichia coli of a rat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase. | Takazawa, K., et al. 1990. Biochem J. 272: 107-12. PMID: 2176078
- Purification of D-myo-inositol 1,4,5-trisphosphate 3-kinase from rat brain. | Johanson, RA., et al. 1988. J Biol Chem. 263: 7465-71. PMID: 2836386
- Tissue- and cell-specific expression of Ins(1,4,5)P3 3-kinase isoenzymes. | Vanweyenberg, V., et al. 1995. Biochem J. 306 (Pt 2): 429-35. PMID: 7887896
- Cyclic GMP modulates depletion-activated Ca2+ entry in pancreatic acinar cells. | Bahnson, TD., et al. 1993. J Biol Chem. 268: 10808-12. PMID: 8098704
- Expression, purification, and regulation of two isoforms of the inositol 1,4,5-trisphosphate 3-kinase. | Woodring, PJ. and Garrison, JC. 1997. J Biol Chem. 272: 30447-54. PMID: 9374536
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IP3KA Antibody (F-3) | sc-271838 | 200 µg/ml | $316.00 | |||
IP3KA Antibody (F-3): m-IgG Fc BP-HRP Bundle | sc-525829 | 200 µg Ab; 10 µg BP | $354.00 | |||
IP3KA Antibody (F-3) AC | sc-271838 AC | 500 µg/ml, 25% agarose | $416.00 | |||
IP3KA Antibody (F-3) HRP | sc-271838 HRP | 200 µg/ml | $316.00 | |||
IP3KA Antibody (F-3) FITC | sc-271838 FITC | 200 µg/ml | $330.00 | |||
IP3KA Antibody (F-3) PE | sc-271838 PE | 200 µg/ml | $343.00 | |||
IP3KA Antibody (F-3) Alexa Fluor® 488 | sc-271838 AF488 | 200 µg/ml | $357.00 | |||
IP3KA Antibody (F-3) Alexa Fluor® 546 | sc-271838 AF546 | 200 µg/ml | $357.00 | |||
IP3KA Antibody (F-3) Alexa Fluor® 594 | sc-271838 AF594 | 200 µg/ml | $357.00 | |||
IP3KA Antibody (F-3) Alexa Fluor® 647 | sc-271838 AF647 | 200 µg/ml | $357.00 | |||
IP3KA Antibody (F-3) Alexa Fluor® 680 | sc-271838 AF680 | 200 µg/ml | $357.00 | |||
IP3KA Antibody (F-3) Alexa Fluor® 790 | sc-271838 AF790 | 200 µg/ml | $357.00 | |||
IP3KA (F-3) Neutralizing Peptide | sc-271838 P | 100 µg/0.5 ml | $68.00 |