Integrin alpha 1 Antibody (A-9)recommended for detection of Integrin α1 of mouse, rat and human origin by WB, IP, IF and ELISA

Integrin α1 Antibody (A-9): sc-271034

Integrin α1 Antibody (A-9) is rated 4.8 out of 5 by 8.
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Datasheets

    • Integrin α1 Antibody (A-9) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 980-1143 mapping at the C-terminus of Integrin α1 (also designated CD49α) of rat origin
    • Integrin alpha 1 Antibody (A-9) is recommended for detection of Integrin α1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Integrin alpha 1 Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Integrin α1 (A-9): sc-271034.
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Can Integrin α1 (A-9): sc-271034 be frozen?

Asked by: jenni
No. The antibody solution contains gelatin. When the gelatin is frozen the antibody precipitates out of solution. However, if an antibody’s datasheet says that the antibody should be stored frozen, then the antibody can be frozen, as these antibodies do not contain gelatin.
Answered by: Technical Support 7
Date published: 2017-01-24

Would it be possible for the Integrin α1 Antibody (A-9) to detect this protein in avian samples?

Asked by: Trav11
To inquire about additional species reactivity, please contact our Technical Service department at 800-457-3801 and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Tech Service 8
Date published: 2016-12-19

What is the recommended secondary reagent to use with the Integrin α1 Antibody (A-9) in Western blot applications?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2016-12-19
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Rated 5 out of 5 by from good feature we used this antibody to detect the expression of integrin in glioma cell lysate by western blot
Date published: 2017-09-25
Rated 5 out of 5 by from Good for IF Use for IF of MDA-MB-231 cells. Seemed to get good signal, not a lot of background.
Date published: 2017-08-17
Rated 5 out of 5 by from great in WB this antibody shows strong and specific signal in WB
Date published: 2017-08-02
Rated 3 out of 5 by from nice I like to try this again to make sure it works or not
Date published: 2017-07-24
Rated 5 out of 5 by from Good cytoplasmic staining Good cytoplasmic staining with paraffin-embedded mouse colon tissue,use dilution 1:400.
Date published: 2017-05-13
Rated 5 out of 5 by from Published for CoIP/WB This Integrin alpha 1 Antibody (A-9) was featured in a recent publication in Nature Communications for use in CoIP and Western blotting applications. PMID: 27542621.
Date published: 2016-12-28
Rated 5 out of 5 by from Great in Western Blot Great in Western Blot.
Date published: 2015-09-28
Rated 5 out of 5 by from Western blot analysis of Integrin 1 expression Western blot analysis of Integrin 1 expression in WI 38 whole cell lysate shows single strong band at target MW -SCBT QC
Date published: 2013-01-16
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