Integrin α1/ITGA1/CD49a Antibody (HM alpha 1) - Western Blotting - Image 380674
Integrin α1/ITGA1/CD49a Double Nickase Plasmid (h): sc-401275-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin α1/ITGA1/CD49a Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin α1/ITGA1/CD49a Double Nickase Plasmid (h) and Integrin α1/ITGA1/CD49a Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ITGA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin α1/ITGA1/CD49a Antibody (A-9): sc-271034
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin α1/ITGA1/CD49a Double Nickase Plasmid (h) | sc-401275-NIC | 20 µg | $410.00 | |||
Integrin α1/ITGA1/CD49a Double Nickase Plasmid (h2) | sc-401275-NIC-2 | 20 µg | $410.00 |