Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IL-8RB Double Nickase Plasmid (h): sc-401404-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-8RB Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-8RB Double Nickase Plasmid (h) and IL-8RB Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CXCR2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-8RB Antibody (E-2): sc-7304
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-8RB Double Nickase Plasmid (h) | sc-401404-NIC | 20 µg | $410.00 | |||
IL-8RB Double Nickase Plasmid (h2) | sc-401404-NIC-2 | 20 µg | $410.00 |
CXCR2 encodes IL-8RB, a G protein–coupled receptor for ELR+ CXC chemokines such as CXCL8/IL-8 that governs neutrophil chemotaxis, adhesion, and activation. Ligand engagement triggers canonical GPCR signaling through Gαi, calcium flux, and downstream PI3K–AKT, MAPK/ERK, and PLCβ pathways, integrating cues that shape inflammatory trafficking and endothelial interactions. IL-8RB is implicated in regulation of innate immune responses, angiogenic and wound-healing programs, and crosstalk with cytokine networks within inflamed tissues. Dysregulated CXCR2 signaling is frequently studied in chronic inflammatory settings and tumor-associated inflammation where myeloid recruitment and chemokine gradients influence microenvironmental remodeling.
IL-8RB Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CXCR2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CXCR2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CXCR2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CXCR2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.