IL-6 Antibody (6D9A1): sc-65989

IL-6 Antibody (6D9A1) is a mouse monoclonal IgM antibody that detects interleukin-6 (IL-6) in human samples through applications such as western blotting (WB) and immunoprecipitation (IP). IL-6 is a multifunctional cytokine composed of 212 amino acids, playing a pivotal role in immune response, host defense, and hematopoiesis. IL-6 is constitutively expressed by epidermal Langerhans cells, with expression further induced in stimulated keratinocytes, highlighting importance in skin immunity and inflammation. The IL-6 receptor is a trimeric complex that includes an IL-6-specific α chain and a homodimer of the gp130 glycoprotein, which is shared among several other interleukin receptors. Upon binding of IL-6, the receptor complex undergoes homodimerization of gp130, leading to activation of Janus kinases JAK1 and JAK2. These kinases subsequently phosphorylate Signal Transducer and Activator of Transcription 3 (Stat3), facilitating translocation to the nucleus where Stat3 drives expression of responsive genes. IL-6 also activates the Ras/MAP kinase pathway, which is crucial for regulating transcription of NFIL6, further underscoring IL-6′s role in inflammatory responses and cellular signaling pathways. Anti-IL-6 antibody (6D9A1) is an essential tool for researchers studying complex mechanisms of immune regulation and pathophysiology of diseases associated with dysregulated IL-6 signaling.

The preferred IL-6 antibody is IL-6 Antibody (E-4): sc-28343.