IL-28R CRISPR/Cas9 KO Plasmid (h): sc-402530
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-28R CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IL-28R genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-28R CRISPR/Cas9 KO Plasmid (h) | sc-402530 | 20 µg | $397.00 |
Overview
IFNLR1 encodes interleukin-28 receptor subunit alpha (IL-28Rα), the ligand-binding component of the type III interferon receptor complex that partners with IL10RB to mediate responses to IFN-λ (IL-28/IL-29). Receptor engagement activates JAK–STAT signaling, promoting STAT1/STAT2–IRF9 (ISGF3) formation and induction of interferon-stimulated genes that shape epithelial and barrier-site innate immunity. IFNLR1 expression is enriched in epithelial tissues, contributing to compartmentalized antiviral and inflammatory signaling distinct from type I interferon pathways. Dysregulated IFN-λ/IFNLR1 signaling has been linked to altered mucosal immune homeostasis and susceptibility to inflammatory and infectious disease phenotypes in respiratory and gastrointestinal contexts.
IL-28R CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the IFNLR1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the IFNLR1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the IFNLR1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IL-28R protein expression.
This CRISPR knockout system enables efficient generation of IFNLR1-deficient cell models for investigation of IL-28R signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting IFNLR1 exon(s) critical for IL-28R function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple IFNLR1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IL-28R CRISPR/Cas9 KO Plasmid (h) and IL-28R CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the IFNLR1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IL-28R HDR Plasmid (h) and IL-28R HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by IFNLR1 homology arms to support homology-directed repair at defined IFNLR1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.