IL-27 Antibody (C-8): sc-390482

IL-27 Antibody (C-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects IL-27 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IL-27 (C-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IL-27 plays a crucial role in the immune response by promoting the expansion of naive CD4+ T cells and guiding their differentiation towards a Th1 phenotype, characterized by the production of gamma-interferon. This function is vital for the development of a robust adaptive immune response, as IL-27 not only influences T cell behavior but also directly affects innate immune cells. Notably, IL-27 levels rise upon the activation of antigen-presenting cells, which enhances signaling capacity. IL-27, consisting of 243 amino acids in humans and showing 73% identity with the mouse counterpart, features an N-terminal signal peptide and several O-glycosylation sites, which are essential for stability and activity. Additionally, a unique stretch of 13 glutamate residues located between helices C and D may play a role in structural integrity and function, further underscoring the importance of precise molecular architecture in mediating immune responses.

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IL-27 Antibody (C-8) References:

1. IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein, induces proliferation of naive CD4+ T cells.  |  Pflanz, S., et al. 2002. Immunity. 16: 779-90. PMID: 12121660
2. IL-23 and IL-27: new members of the growing family of IL-12-related cytokines with important implications for therapeutics.  |  Cordoba-Rodriguez, R. and Frucht, DM. 2003. Expert Opin Biol Ther. 3: 715-23. PMID: 12880372
3. IL-27 regulates IL-12 responsiveness of naive CD4+ T cells through Stat1-dependent and -independent mechanisms.  |  Lucas, S., et al. 2003. Proc Natl Acad Sci U S A. 100: 15047-52. PMID: 14657353
4. Understanding the pro- and anti-inflammatory properties of IL-27.  |  Villarino, AV., et al. 2004. J Immunol. 173: 715-20. PMID: 15240655
5. Suppression of ongoing adjuvant-induced arthritis by neutralizing the function of the p28 subunit of IL-27.  |  Goldberg, R., et al. 2004. J Immunol. 173: 1171-8. PMID: 15240707
6. Induction of IgG2a class switching in B cells by IL-27.  |  Yoshimoto, T., et al. 2004. J Immunol. 173: 2479-85. PMID: 15294962
7. The IL-27 receptor (WSX-1) is an inhibitor of innate and adaptive elements of type 2 immunity.  |  Artis, D., et al. 2004. J Immunol. 173: 5626-34. PMID: 15494513
8. IL-27-induced modulation of autoimmunity and its therapeutic potential.  |  Meka, RR., et al. 2015. Autoimmun Rev. 14: 1131-1141. PMID: 26253381
9. The role of the cytokines IL-27 and IL-35 in cancer.  |  Friedman, A. and Liao, KL. 2015. Math Biosci Eng. 12: 1203-17. PMID: 26775857
10. IL-27-containing exosomes secreted by innate B-1a cells suppress and ameliorate uveitis.  |  Kang, M., et al. 2023. Front Immunol. 14: 1071162. PMID: 37334383
11. IL-27 expression regulation and its effects on adaptive immunity against viruses.  |  Andres-Martin, F., et al. 2024. Front Immunol. 15: 1395921. PMID: 38966644
12. IL-27-engineered CAR.19-NK-92 cells exhibit enhanced therapeutic efficacy.  |  Biggi, AFB., et al. 2024. Cytotherapy. 26: 1320-1330. PMID: 38970613

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IL-27 Antibody (C-8) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-IL-27 Antibody (C-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IL-27 Antibody (C-8) Specifications

Alternate Product Name: IL-27

Product Clone Name: C-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 91-105

Epitope Target: IL-27

Epitope Species: mouse

Product Reactivity/Detection: IL-27

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for IL-27 Antibody (C-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred IL-27 antibody is IL-27 Antibody (LB-L22): sc-134367.