IL-1RII CRISPR/Cas9 KO Plasmid (m2): sc-421099-KO-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-1RII CRISPR/Cas9 Knockout (KO) Plasmid (m2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IL-1RII genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-1RII CRISPR/Cas9 KO Plasmid (m2) | sc-421099-KO-2 | 20 µg | $397.00 |
Overview
Il1r2 encodes mouse interleukin-1 receptor type II (IL-1RII), a high-affinity decoy receptor that binds IL-1α and IL-1β and dampens proinflammatory signaling by limiting ligand availability for IL-1R1/IL-1RAcP signal transduction. By attenuating IL-1–driven activation of NF-κB and MAPK pathways, IL-1RII helps shape cytokine networks, leukocyte recruitment, and innate immune activation in myeloid and stromal compartments. Altered regulation of IL-1RII has been linked to inflammatory and autoimmune phenotypes through effects on cytokine balance and tissue inflammatory tone. Il1r2 is therefore used to investigate mechanisms controlling IL-1 responsiveness, decoy receptor biology, and resolution versus persistence of inflammation in mouse models.
IL-1RII CRISPR/Cas9 KO Plasmid (m2) is a pool of plasmids designed for targeted disruption of the Il1r2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Il1r2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Il1r2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IL-1RII protein expression.
This CRISPR knockout system enables efficient generation of Il1r2-deficient cell models for investigation of IL-1RII signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Il1r2 exon(s) critical for IL-1RII function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Il1r2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IL-1RII CRISPR/Cas9 KO Plasmid (m) and IL-1RII CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Il1r2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IL-1RII HDR Plasmid (m) and IL-1RII HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Il1r2 homology arms to support homology-directed repair at defined Il1r2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.