IL-13Rα1 (h): 293T Lysate: sc-175871
- human IL-13Rα1 transfected 293T lysate
- 100 µg protein in 200 µl SDS-PAGE buffer; suitable as Western Blotting control for IL-13Rα1 antibodies
- Suitable control antibody: IL-13Rα1 Antibody (D-2): sc-398831 is recommended as control antibody for monitoring of IL-13Rα1 expression knockdown by Western blotting or immunofluorescence
- should be stored at -20° C and repeated freezing and thawing should be minimized
- sample vial should be boiled once prior to use
- non-transfected control 293T lysate (sc-117752)
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IL-13Rα1 (h): 293T Lysate is derived from the HEK 293T cell line and is essential for studying the Interleukin-13 Receptor Alpha 1 (IL-13Rα1) in human cells, focusing on its role in cellular signaling and molecular biology. This lysate facilitates research into the mechanisms of IL-13Rα1 as part of the receptor complex that interacts with the cytokine IL-13, a process central to understanding signaling pathways involved in cellular functions. The 293T cells, modified from the original HEK 293 line to express the SV40 large T antigen, enhance the replication and expression of recombinant DNA, making them ideal for high-level protein expression. Researchers utilize this lysate to investigate how IL-13Rα1 contributes to the signaling cascade that influences various cellular responses, including gene expression modulation and pathway activation, through methods such as co-immunoprecipitation, flow cytometry, and luciferase reporter assays. This detailed examination helps in elucidating the receptor's role in signal transduction mechanisms, focusing on how it regulates cellular functions through its interaction with IL-13, all within the realm of fundamental scientific inquiry. This research contributes to a broader understanding of cytokine-receptor interactions and their implications in cell biology.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-13Rα1 (h): 293T Lysate | sc-175871 | 100 µg/200 µl | $232.00 |