IL-10Rα Double Nickase Plasmid (h): sc-402486-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-10Rα Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-10Rα Double Nickase Plasmid (h) and IL-10Rα Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IL10RA. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-10Rα Antibody (A-3): sc-365374
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-10Rα Double Nickase Plasmid (h) | sc-402486-NIC | 20 µg | $410.00 | |||
IL-10Rα Double Nickase Plasmid (h2) | sc-402486-NIC-2 | 20 µg | $410.00 |
IL10RA encodes the alpha chain of the interleukin-10 receptor (IL-10Rα), a high-affinity binding component required for IL-10–dependent anti-inflammatory signaling. Upon ligand engagement, IL-10Rα pairs with IL10RB to activate JAK1/TYK2 and downstream STAT3 transcriptional programs that limit pro-inflammatory cytokine production and modulate antigen-presenting cell function. This pathway shapes myeloid and lymphoid immune homeostasis at mucosal and systemic sites, influencing macrophage polarization, dendritic cell maturation, and T cell responses. Genetic or functional perturbation of IL10RA is associated with immune dysregulation and inflammatory disease phenotypes, supporting its relevance in studies of cytokine signaling, host–microbiome interactions, and barrier immunity.
IL-10Rα Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IL10RA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IL10RA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IL10RA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IL10RA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.