IGFBP6 Double Nickase Plasmid (h): sc-404768-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IGFBP6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IGFBP6 Double Nickase Plasmid (h) and IGFBP6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IGFBP6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IGFBP6 Antibody (1A8): sc-293295
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IGFBP6 Double Nickase Plasmid (h) | sc-404768-NIC | 20 µg | $410.00 | |||
IGFBP6 Double Nickase Plasmid (h2) | sc-404768-NIC-2 | 20 µg | $410.00 |
IGFBP6 encodes insulin-like growth factor binding protein 6, a secreted regulator with high affinity for IGF-II that modulates IGF bioavailability and downstream IGF1R-driven signaling. By buffering extracellular IGF-II, IGFBP6 influences PI3K–AKT and MAPK pathway activity, affecting cell proliferation, survival, and differentiation programs. IGFBP6 has also been linked to migration and extracellular matrix interactions, supporting roles in tissue remodeling and cell–microenvironment crosstalk. Altered IGFBP6 expression has been reported across multiple cancer and metabolic research contexts, making it a relevant node for studying IGF axis dysregulation and growth factor–dependent phenotypes.
IGFBP6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IGFBP6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IGFBP6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IGFBP6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IGFBP6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.