IGFBP4 CRISPR/Cas9 KO Plasmid (m): sc-421065
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IGFBP4 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IGFBP4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IGFBP4 CRISPR/Cas9 KO Plasmid (m) | sc-421065 | 20 µg | $397.00 |
Overview
Igfbp4 encodes insulin-like growth factor binding protein 4 (IGFBP4), a secreted regulator of IGF bioavailability that modulates IGF1/IGF2 interactions with IGF receptors and thereby influences downstream PI3K–AKT and MAPK signaling. In mouse tissues, IGFBP4 helps shape cell proliferation, survival, differentiation, and extracellular microenvironment signaling through control of local IGF activity. Altered IGFBP4 expression has been associated with remodeling and inflammatory programs in vascular and stromal compartments and with changes in growth factor–driven phenotypes relevant to metabolic regulation and tumor-associated microenvironments. As a context-dependent modulator of mitogenic signaling, Igfbp4 is frequently investigated in studies of development, tissue homeostasis, and disease mechanisms involving dysregulated growth factor signaling.
IGFBP4 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Igfbp4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Igfbp4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Igfbp4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IGFBP4 protein expression.
This CRISPR knockout system enables efficient generation of Igfbp4-deficient cell models for investigation of IGFBP4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Igfbp4 exon(s) critical for IGFBP4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Igfbp4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IGFBP4 CRISPR/Cas9 KO Plasmid (m) and IGFBP4 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Igfbp4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IGFBP4 HDR Plasmid (m) and IGFBP4 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Igfbp4 homology arms to support homology-directed repair at defined Igfbp4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.